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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MGVILQDQVA LVTGASRGIG RGIALQLGEA GATVYITGRR PELSDNFRLG LPSLDYVAKE

70 80 90 100 110 120
ITSRGGKGIA LYVDHSNMTE VKFLFEKIKE DEEGKLDILV NNVYNSLGKA TEMIGKTFFD

130 140 150 160 170 180
QDPSFWDDIN GVGLRNHYYC SVYAARMMVE RRKGLIVNVG SLGGLKYVFN VAYGAGKEAL

190 200 210 220 230 240
ARMSTDMAVE LNPYNVCVVT LIPGPVKTET ANRTIIDDAY KMIKENPELE EFIKGESTEY

250 260 270 280 290 300
TGKALARLAM DPGKLKKSGK TLFTEDLAQK YDFSDKHGME PQNIRSIRTI LGTMGKEEVA

310 320
KYIPPQIKLP KWVIWQSVNR F


References and documentation are available.

Number of amino acids: 321 Molecular weight: 35696.13 Theoretical pI: 8.28
Amino acid composition: 
Ala (A) 22 6.9% Arg (R) 16 5.0% Asn (N) 15 4.7% Asp (D) 17 5.3% Cys (C) 2 0.6% Gln (Q) 8 2.5% Glu (E) 23 7.2% Gly (G) 31 9.7% His (H) 3 0.9% Ile (I) 22 6.9% Leu (L) 28 8.7% Lys (K) 26 8.1% Met (M) 11 3.4% Phe (F) 11 3.4% Pro (P) 12 3.7% Ser (S) 15 4.7% Thr (T) 18 5.6% Trp (W) 3 0.9% Tyr (Y) 14 4.4% Val (V) 24 7.5% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 40 Total number of positively charged residues (Arg + Lys): 42 Atomic composition: Carbon C 1597 Hydrogen H 2545 Nitrogen N 427 Oxygen O 472 Sulfur S 13 Formula: C1597H2545N427O472S13 Total number of atoms: 5054 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 37485 Abs 0.1% (=1 g/l) 1.050, assuming all pairs of Cys residues form cystines Ext. coefficient 37360 Abs 0.1% (=1 g/l) 1.047, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 31.96 This classifies the protein as stable. Aliphatic index: 89.28 Grand average of hydropathicity (GRAVY): -0.243