ProtParam
User-provided sequence:
10 20 30 40 50 60
MGVILQDQVA LVTGASRGIG RGIALQLGEA GATVYITGRR PELSDNFRLG LPSLDYVAKE
70 80 90 100 110 120
ITSRGGKGIA LYVDHSNMTE VKFLFEKIKE DEEGKLDILV NNVYNSLGKA TEMIGKTFFD
130 140 150 160 170 180
QDPSFWDDIN GVGLRNHYYC SVYAARMMVE RRKGLIVNVG SLGGLKYVFN VAYGAGKEAL
190 200 210 220 230 240
ARMSTDMAVE LNPYNVCVVT LIPGPVKTET ANRTIIDDAY KMIKENPELE EFIKGESTEY
250 260 270 280 290 300
TGKALARLAM DPGKLKKSGK TLFTEDLAQK YDFSDKHGME PQNIRSIRTI LGTMGKEEVA
310 320
KYIPPQIKLP KWVIWQSVNR F
References and
documentation are available.
Number of amino acids: 321
Molecular weight: 35696.13
Theoretical pI: 8.28
Total number of negatively charged residues (Asp + Glu): 40
Total number of positively charged residues (Arg + Lys): 42
Atomic composition:
Carbon C 1597
Hydrogen H 2545
Nitrogen N 427
Oxygen O 472
Sulfur S 13
Formula: C
1597H
2545N
427O
472S
13
Total number of atoms: 5054
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 37485
Abs 0.1% (=1 g/l) 1.050, assuming all pairs of Cys residues form cystines
Ext. coefficient 37360
Abs 0.1% (=1 g/l) 1.047, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 31.96
This classifies the protein as stable.
Aliphatic index: 89.28
Grand average of hydropathicity (GRAVY): -0.243