ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MGVKHNPKRD RHIDAARAAG SRFGAETLPP EILFGGASND DLDRYTPEML ALTAAHARGE 

        70         80         90        100        110        120 
LARWDGGKPQ VSVETVAGVA PGGIEVSIIA ITERNMPFLY DSVMGEVTST HRDIHLAIHP 

       130        140        150        160        170        180 
ILVMEPGKPV KLFDPDEESA PEHRVSHIQI HLSRLTPLEA RSLSKRISDV LEQVHQAVHD 

       190        200        210        220        230        240 
WPAMTALLDQ AMRELEDYNA SRKKSDRDEA LAFLRWLRDS NFTFLGMREY TYSGKGGRAT 

       250        260        270        280        290        300 
VERGKGRGLG ILSNPDVRVL RQGKDAVLTT PEILAFLEGP DFLIVTKANV KSVVHRRAHM 

       310        320        330        340        350        360 
DYIGIKRFDA SGNVVGELRI VGLFTSTAYT RQASEIPLLR HKIEKIVDHF GYDPQSHSGK 

       370        380        390        400        410        420 
TLANTLEAYP RDDLFQIDVG LLAAFCEQIN ELGDRPRVRV LPRIDHFDRF VSVIVFVPRE 

       430        440        450        460        470        480 
QYDSDVREKI GDYLKTVYDG RVSAYYPAFP EGGLARVHFI IGRSGGKTPR VPQAKLEEAV 

       490        500        510        520        530        540 
RAIVTRWIDR FNLLARKEGT EISVGEAYQA AFTPAEAYAD LGDIAACTAG DPIRISFYHR 

       550        560        570        580        590        600 
HQERPDTLEL KIFHADTPVS LSRRVPLLEN LGFRVISEQT YDIGVHVHGD EPREVVLHDM 

       610        620        630        640        650        660 
ELIHRDGHTL NLARIGPALE EAFLAAWNGT TEDDNFNRLV LLAGLTAREI TVLRAYARYL 

       670        680        690        700        710        720 
RQAGITYSQG YIADTLNKYP TIAADIFRLF STRMDPTTEV KARTKKCSAL LTGIEEALSA 

       730        740        750        760        770        780 
VPSLDEDRIL RRYVNAVQST LRTNYFQKDA EGRPRAVLAF KLDPKQLEGL PEPRPFREIF 

       790        800        810        820        830        840 
VYGTEVEGVH LRFGKVARGG LRWSDRAQDY RTEVLGLVKA QQVKNAVIVP VGAKGGFYPK 

       850        860        870        880        890        900 
QLPVGGSRDE IFKAGTEAYK TYIRTLLSVT DNIIGQEVVP PEDTLRLDGD DPYFVVAADK 

       910        920        930        940        950        960 
GTATFSDTAN ALAQEADFWL DDAFASGGSA GYDHKKMGIT GRGAWEAVKR HFREMDVDIQ 

       970        980        990       1000       1010       1020 
TTPFTVAGVG DMSGDVFGNG MLLSEKIRLI AAFDHRDIFI DPEPDIDLSF GERKRMFALP 

      1030       1040       1050       1060       1070       1080 
RSSWQDYDRK ALSPGAMIIS RSEKLVTLTP EAMAAIGIDK PKATPFEIMS AILKSPVDLL 

      1090       1100       1110       1120       1130       1140 
WFGGIGTYVR GANETDAEVG DRANDAIRVS AEDVRARVIG EGANLGVTQK GRIGFSLNGG 

      1150       1160       1170       1180       1190       1200 
RCNSDAIDNS AGVNSSDVEV NIKIALASAM RDDRLTRPKR NTLLASMTDE VGHLVLRNNY 

      1210       1220       1230       1240       1250       1260 
QQSLAISLTE MQGLANRTPL ARLMARLEAD GHLNRKVETL PTDQAMSERY QAGRPLTRPE 

      1270       1280       1290       1300       1310       1320 
IGVLLSYAKL VLFDELIVSE IPDDPYFTAT LERYFPAKMR KAYAGDIHGH RLRREIIATV 

      1330       1340       1350       1360       1370       1380 
LANETINRGG PAFVSTLTDA TGFLSADVVK AAVLALDGFD LPRIYGEIDA LDNRISGAIQ 

      1390       1400       1410       1420       1430       1440 
NRLYHEVGRI FALVAERALR TRASEGSVAE AVARLRDGLQ KLRGTMRAAI SREGAEEARL 

      1450       1460       1470       1480       1490       1500 
RAAGFIENGV SAKLAKEIAE LSLMTLVPEI MQIATVTGEP LSRTAQAYFT VTESLRINRL 

      1510       1520       1530       1540       1550       1560 
LAAADRVPAT EQFESMALSR AVGDIGTARR DITIAALVEH KGDRNPVLAW QDRDRQRVAT 

      1570       1580       1590 
VGDQLRLLTE KGETTLAKVT VAAGLLSDLA RGWTK 

References and documentation are available.

Number of amino acids: 1595

Molecular weight: 176256.56

Theoretical pI: 6.32

Amino acid composition: 
Ala (A) 173	 10.8%
Arg (R) 141	  8.8%
Asn (N)  43	  2.7%
Asp (D) 111	  7.0%
Cys (C)   4	  0.3%
Gln (Q)  42	  2.6%
Glu (E) 106	  6.6%
Gly (G) 124	  7.8%
His (H)  36	  2.3%
Ile (I)  94	  5.9%
Leu (L) 154	  9.7%
Lys (K)  63	  3.9%
Met (M)  29	  1.8%
Phe (F)  59	  3.7%
Pro (P)  71	  4.5%
Ser (S)  80	  5.0%
Thr (T)  96	  6.0%
Trp (W)  12	  0.8%
Tyr (Y)  43	  2.7%
Val (V) 114	  7.1%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 217
Total number of positively charged residues (Arg + Lys): 204

Atomic composition:

Carbon      C	      7807
Hydrogen    H	     12472
Nitrogen    N	      2250
Oxygen      O	      2334
Sulfur      S	        33

Formula: C7807H12472N2250O2334S33
Total number of atoms: 24896

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   130320
Abs 0.1% (=1 g/l)   0.739, assuming all pairs of Cys residues form cystines


Ext. coefficient   130070
Abs 0.1% (=1 g/l)   0.738, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 36.55
This classifies the protein as stable.



Aliphatic index: 92.21

Grand average of hydropathicity (GRAVY): -0.241