ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MGVLVISLLV VHLLAFSVCV QGGYRRGGHH PGGHMGPWIN AHATFYGGGD ASGTMGGACG 

        70         80         90        100        110        120 
YGNLYSQGYG LETAALSTAL FDQGLSCGAC FELMCVNDPQ WCIKGRSIVV TATNFCPPGG 

       130        140        150        160        170        180 
ACDPPNHHFD LSQPIYEKIA LYKSGIIPVM YRRVRCKRSG GIRFTINGHS YFNLVLVTNV 

       190        200        210        220        230        240 
GGAGDVHSVS MKGSRTKWQL MSRNWGQNWQ SNSYLNGQSL SFVVTTSDRR SVVSFNVAPP 

       250 
TWSFGQTYTG GQFRY 

References and documentation are available.

Number of amino acids: 255

Molecular weight: 27611.40

Theoretical pI: 9.20

Amino acid composition: 
Ala (A)  14	  5.5%
Arg (R)  13	  5.1%
Asn (N)  13	  5.1%
Asp (D)   7	  2.7%
Cys (C)   9	  3.5%
Gln (Q)  11	  4.3%
Glu (E)   3	  1.2%
Gly (G)  36	 14.1%
His (H)   9	  3.5%
Ile (I)  10	  3.9%
Leu (L)  18	  7.1%
Lys (K)   6	  2.4%
Met (M)   7	  2.7%
Phe (F)  12	  4.7%
Pro (P)  11	  4.3%
Ser (S)  23	  9.0%
Thr (T)  14	  5.5%
Trp (W)   6	  2.4%
Tyr (Y)  12	  4.7%
Val (V)  21	  8.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 10
Total number of positively charged residues (Arg + Lys): 19

Atomic composition:

Carbon      C	      1229
Hydrogen    H	      1864
Nitrogen    N	       348
Oxygen      O	       349
Sulfur      S	        16

Formula: C1229H1864N348O349S16
Total number of atoms: 3806

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    51380
Abs 0.1% (=1 g/l)   1.861, assuming all pairs of Cys residues form cystines


Ext. coefficient    50880
Abs 0.1% (=1 g/l)   1.843, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 35.97
This classifies the protein as stable.



Aliphatic index: 72.20

Grand average of hydropathicity (GRAVY): -0.058