ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MGVSLLASSS AFVTKPLLTQ LVHLSPISLN FTVRRFKPFT CLSRYYTTNP YNMTSNFKTF 

        70         80         90        100        110        120 
NLDFLKPDLD ERSYRFIELP NKLKALLIQD PKADKAAASL DVNIGAFEDP KNLPGLAHFC 

       130        140        150        160        170        180 
EHLLFMGSEK FPDENEYSSY LSKHGGSSNA YTASQNTNYF FEVNHQHLFG ALDRFSGFFS 

       190        200        210        220        230        240 
CPLFNKDSTD KEINAVNSEN KKNLQNDIWR IYQLDKSLTN TKHPYHKFST GNIETLGTLP 

       250        260        270        280        290        300 
KENGLNVRDE LLKFHKNFYS ANLMKLCILG REDLDTLSDW TYDLFKDVAN NGREVPLYAE 

       310        320        330        340        350        360 
PIMQPEHLQK IIQVRPVKDL KKLEISFTVP DMEEHWESKP PRILSHLIGH EGSGSLLAHL 

       370        380        390        400        410        420 
KKLGWANELS AGGHTVSKGN AFFAVDIDLT DNGLTHYRDV IVLIFQYIEM LKNSLPQKWI 

       430        440        450        460        470        480 
FNELQDISNA TFKFKQAGSP SSTVSSLAKC LEKDYIPVSR ILAMGLLTKY EPDLLTQYTD 

       490        500        510        520        530        540 
ALVPENSRVT LISRSLETDS AEKWYGTAYK VVDYPADLIK NMKSPGLNPA LTLPRPNEFV 

       550        560        570        580        590        600 
STNFKVDKID GIKPLDEPVL LLSDDVSKLW YKKDDRFWQP RGYIYLSFKL PHTHASIINS 

       610        620        630        640        650        660 
MLSTLYTQLA NDALKDVQYD AACADLRISF NKTNQGLAIT ASGFNEKLII LLTRFLQGVN 

       670        680        690        700        710        720 
SFEPKKDRFE ILKDKTIRHL KNLLYEVPYS QMSNYYNAII NERSWSTAEK LQVFEKLTFE 

       730        740        750        760        770        780 
QLINFIPTIY EGVYFETLIH GNIKHEEALE VDSLIKSLIP NNIHNLQVSN NRLRSYLLPK 

       790        800        810        820        830        840 
GKTFRYETAL KDSQNVNSCI QHVTQLDVYS EDLSALSGLF AQLIHEPCFD TLRTKEQLGY 

       850        860        870        880        890        900 
VVFSSSLNNH GTANIRILIQ SEHTTPYLEW RINNFYETFG QVLRDMPEED FEKHKEALCN 

       910        920        930        940        950        960 
SLLQKFKNMA EESARYTAAI YLGDYNFTHR QKKAKLVANI TKQQMIDFYE NYIMSENASK 

       970        980        990       1000       1010       1020 
LILHLKSQVE NKELNENELD TAKYPTGQLI EDVGAFKSTL FVAPVRQPMK DFEISAPPKL 


NNSSESE 

References and documentation are available.

Number of amino acids: 1027

Molecular weight: 117578.84

Theoretical pI: 6.27

Amino acid composition: 
Ala (A)  57	  5.6%
Arg (R)  34	  3.3%
Asn (N)  73	  7.1%
Asp (D)  57	  5.6%
Cys (C)   9	  0.9%
Gln (Q)  37	  3.6%
Glu (E)  68	  6.6%
Gly (G)  40	  3.9%
His (H)  29	  2.8%
Ile (I)  59	  5.7%
Leu (L) 126	 12.3%
Lys (K)  80	  7.8%
Met (M)  16	  1.6%
Phe (F)  56	  5.5%
Pro (P)  47	  4.6%
Ser (S)  82	  8.0%
Thr (T)  58	  5.6%
Trp (W)  10	  1.0%
Tyr (Y)  44	  4.3%
Val (V)  45	  4.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 125
Total number of positively charged residues (Arg + Lys): 114

Atomic composition:

Carbon      C	      5319
Hydrogen    H	      8247
Nitrogen    N	      1387
Oxygen      O	      1572
Sulfur      S	        25

Formula: C5319H8247N1387O1572S25
Total number of atoms: 16550

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   121060
Abs 0.1% (=1 g/l)   1.030, assuming all pairs of Cys residues form cystines


Ext. coefficient   120560
Abs 0.1% (=1 g/l)   1.025, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 33.98
This classifies the protein as stable.



Aliphatic index: 88.51

Grand average of hydropathicity (GRAVY): -0.388