ProtParam
User-provided sequence:
10 20 30 40 50 60
MGVTSGGLNF KDTVFNEQQR DIESTTTQVE NQDVFFLTLL VQTVSNGSGG RFVNNTQDIQ
70 80 90 100 110 120
TSNGTSILGS LSLRIVEVSW DSDDSVIDLG SQVRFGSFLH LTQDHGGDLF WGKVLGFTLK
130 140 150 160 170 180
FNLNLRLTVN IDQLEWEVLH VSLHFWVVEV STDQTLSVEN GIRRIHSSLI LSSITNQSFS
190 200 210
VSESDKRWSG SVTLIVGNNV HTIISKVSNT RVCCT
References and
documentation are available.
Number of amino acids: 215
Molecular weight: 23811.62
Theoretical pI: 5.10
Total number of negatively charged residues (Asp + Glu): 22
Total number of positively charged residues (Arg + Lys): 14
Atomic composition:
Carbon C 1050
Hydrogen H 1655
Nitrogen N 291
Oxygen O 335
Sulfur S 3
Formula: C
1050H
1655N
291O
335S
3
Total number of atoms: 3334
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 27625
Abs 0.1% (=1 g/l) 1.160, assuming all pairs of Cys residues form cystines
Ext. coefficient 27500
Abs 0.1% (=1 g/l) 1.155, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 25.81
This classifies the protein as stable.
Aliphatic index: 99.02
Grand average of hydropathicity (GRAVY): -0.033