ProtParam
User-provided sequence:
10 20 30 40 50 60
MGYLFEETLS SNPKTPIVVD DDNELGLMAV RLANAAAFPM VLKASLELGV FDTLYAEASR
70 80 90 100 110 120
TDSFLSPSEI ASKLPTTPRN PGAPVLLDRM LRLLASYSMV KCEKVSVGKE QRVYRAEPIC
130 140 150 160 170 180
RFFLKNNIQD IGSLASQVIV NFDSVFLNTW AQLKDVVLEG GDAFGRAHGG MKLFDYMGTD
190 200 210 220 230 240
ERFSKLFNQT GFTIAVVKKA LEVYQGFKGV NVLVDVGGGV GNTLGVVTSK YPNIKGINFD
250 260 270 280 290 300
LTCALAQAPT YPGVEHVAGD MFVDVPTGNA MILKRILHDW TDEDCVKILK NCWKSLPQNG
310 320 330 340 350 360
KVVVIELVTP DEAENGDINA NIAFDMDMLM FTQCSGGKER SRAEFEALAA ASGFSHCQFV
370
CQAYHCWIIE FCK
References and
documentation are available.
Number of amino acids: 373
Molecular weight: 40935.12
Theoretical pI: 5.20
Total number of negatively charged residues (Asp + Glu): 44
Total number of positively charged residues (Arg + Lys): 35
Atomic composition:
Carbon C 1832
Hydrogen H 2868
Nitrogen N 478
Oxygen O 540
Sulfur S 22
Formula: C
1832H
2868N
478O
540S
22
Total number of atoms: 5740
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 36035
Abs 0.1% (=1 g/l) 0.880, assuming all pairs of Cys residues form cystines
Ext. coefficient 35410
Abs 0.1% (=1 g/l) 0.865, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 23.89
This classifies the protein as stable.
Aliphatic index: 90.16
Grand average of hydropathicity (GRAVY): 0.064