ProtParam
User-provided sequence:
10 20 30 40 50 60
MGYLLEETLS SNSKTPIVID DDNELGLMAV RLANAAAFPM VLKAALELGV FDTLYAEASR
70 80 90 100 110 120
SDSFLSPSEI ASKLPTTPRN PEAPVLLDRM LRLLASYSVV KCGKVSEGKG ERVYRAEPIC
130 140 150 160 170 180
RFFLKDNIQD IGSLASQVIV NFDSVFLNTW AQLKDVVLEG GDAFGRAHGG MKLFDYMGTD
190 200 210 220 230 240
ERFSKLFNQT GFTIAVVKKA LEVYQGFKGV NVLVDVGGGV GNTLGVVASK YPNIKGINFD
250 260 270 280 290 300
LTCALAQAPS YPGVEHVAGD MFVDVPTGDA MILKRILHDW TDEDCVKILK NCWKSLPESG
310 320 330 340 350 360
KVVVIELVTP DEAENGDINA NIAFDMDMLM FTQCSGGKER SRAEFEALAA ASGFTHCKFV
370
CQAYHCWIIE FCK
References and
documentation are available.
Number of amino acids: 373
Molecular weight: 40747.86
Theoretical pI: 5.01
Total number of negatively charged residues (Asp + Glu): 48
Total number of positively charged residues (Arg + Lys): 36
Atomic composition:
Carbon C 1823
Hydrogen H 2859
Nitrogen N 473
Oxygen O 542
Sulfur S 21
Formula: C
1823H
2859N
473O
542S
21
Total number of atoms: 5718
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 36035
Abs 0.1% (=1 g/l) 0.884, assuming all pairs of Cys residues form cystines
Ext. coefficient 35410
Abs 0.1% (=1 g/l) 0.869, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 28.06
This classifies the protein as stable.
Aliphatic index: 92.01
Grand average of hydropathicity (GRAVY): 0.083