ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MHCLCVLRST LCCQMPTSTY RMLLQTKAVP VQIIKRDLTA KVKNQKLNQN IRRVDRRNAA 

        70         80         90        100        110        120 
YLLRHTTWKD LVELYEIAKP FRKWIFLGTS FMVVSSGIFL IIPRILGKLI DQFDSNTGVQ 

       130        140        150        160        170        180 
KAEEVFTMKI AKYFKEQPMS LVGLLAIGAL ATAAKVYCLQ IAGVNIVGSL RKMVYSSIIR 

       190        200        210        220        230        240 
QDMTFFDKNM VGEIGSRHAA DTVIVGYSVS SSLRAGVRAV LVGVGSIGMM LMTSLELSTV 

       250        260        270        280        290        300 
SFLTAPIVMG IFKMFGRVQQ QCTWQLQEVA ADVNQTAIER MANMKTIRML SAEQQSIDEY 

       310        320        330        340        350        360 
CKKGELVLDI SKTEALAKGS LIGSFQFTGY SAFSSILFYG SHLINTGHIT YGELSSFCLY 

       370        380        390        400        410        420 
AVLAAASLSN ISGFYNEVMK GLGASSRLLE LKNAQPIMNL NSGIKKTDIT TSIQFENVSF 

       430        440        450        460        470        480 
AYAGRKKTLE NISFDIPRGK ITAVIGPSGS GKSSIANLML RLYDPADGRV TVDGVDLRDL 

       490        500        510        520        530        540 
NATTWRHAIG TVGQEPVLFT CSIRENILMG TEHPEKVSQY ELEEAAQLAN ALDFIQGFEN 

       550        560        570        580        590        600 
GFETMVGEHG CKLSGGQKQR IAIARALISK PKIMILDEAT SALDATSDYL IRMTLDNLLK 

       610        620        630        640        650        660 
NHNLTVLVIA HRLATMQQAD QVVYVNRGRI EGQGTFDEMM KIQNSIVEQE ARRRGIPMNE 

       670 
QMSRVNTPVA RGFSLKF 

References and documentation are available.

Number of amino acids: 677

Molecular weight: 74926.20

Theoretical pI: 9.25

Amino acid composition: 
Ala (A)  54	  8.0%
Arg (R)  37	  5.5%
Asn (N)  29	  4.3%
Asp (D)  25	  3.7%
Cys (C)  10	  1.5%
Gln (Q)  34	  5.0%
Glu (E)  34	  5.0%
Gly (G)  49	  7.2%
His (H)  10	  1.5%
Ile (I)  54	  8.0%
Leu (L)  67	  9.9%
Lys (K)  38	  5.6%
Met (M)  29	  4.3%
Phe (F)  28	  4.1%
Pro (P)  15	  2.2%
Ser (S)  52	  7.7%
Thr (T)  42	  6.2%
Trp (W)   4	  0.6%
Tyr (Y)  18	  2.7%
Val (V)  48	  7.1%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 59
Total number of positively charged residues (Arg + Lys): 75

Atomic composition:

Carbon      C	      3324
Hydrogen    H	      5385
Nitrogen    N	       913
Oxygen      O	       971
Sulfur      S	        39

Formula: C3324H5385N913O971S39
Total number of atoms: 10632

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    49445
Abs 0.1% (=1 g/l)   0.660, assuming all pairs of Cys residues form cystines


Ext. coefficient    48820
Abs 0.1% (=1 g/l)   0.652, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 33.17
This classifies the protein as stable.



Aliphatic index: 98.24

Grand average of hydropathicity (GRAVY): 0.058