ProtParam
User-provided sequence:
10 20 30 40 50 60
MHCPICHHRA HVVYCAHCIN TSPSLLLKLK LDLILLKDEN KELNGKVEQI LNEAMNYDQL
70 80 90 100 110 120
DIKRMEKKKD PLMNSLMKLD VLRMKKNNNL IRHRIEQLNE RIYSKRNHIS ELKVEIDNYK
130 140 150 160 170 180
CYKVGTGTDK LREQVEISDA KNKLAQVSKI CESARDYKLN LLNNWFVIQK LQDNFQIPFA
190 200 210 220 230 240
IAFQPLISLK NFRILPLAIT NDSINIMWKY ISFFSDILMI KLPYTNKICE QPMFEFSDSI
250 260 270 280 290 300
QTVVQRLIKL IINILQICRH LKLVPSTPMD IPWLLDQYDV DGLFYNMVKR NKMKCRSVSL
310 320 330 340
YWTFGMLYSM VLDNMNNPQR GHPARRTAPP PTVTGPHDRW YVVG
References and
documentation are available.
Number of amino acids: 344
Molecular weight: 40453.61
Theoretical pI: 9.33
Total number of negatively charged residues (Asp + Glu): 34
Total number of positively charged residues (Arg + Lys): 49
Atomic composition:
Carbon C 1819
Hydrogen H 2914
Nitrogen N 496
Oxygen O 497
Sulfur S 24
Formula: C
1819H
2914N
496O
497S
24
Total number of atoms: 5750
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 47370
Abs 0.1% (=1 g/l) 1.171, assuming all pairs of Cys residues form cystines
Ext. coefficient 46870
Abs 0.1% (=1 g/l) 1.159, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 39.74
This classifies the protein as stable.
Aliphatic index: 100.84
Grand average of hydropathicity (GRAVY): -0.282