ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MHEMQGILLC SGGGTRMPVL TRHVQKCLLP VVGVPMFLYP LSSLLRTGIT DIKIFVREVL 

        70         80         90        100        110        120 
QLTLEKEVKK SKLLEKYPAH IEYICVNQED FGTADLLKNH HSKITKDALI VSCDFISDAS 

       130        140        150        160        170        180 
LIPLVDFFRA TNSTLVALIA DTCVNAPAPG SKSKKPKATD VMAIVESTGQ LAFLCGDDDF 

       190        200        210        220        230        240 
DAPLVMEKSL KIFPSIKLTS KYNDCHVYAI RHKVLLNLSK SKHISSFKAD FVPLLIDKQF 

       250        260        270        280        290        300 
EPDSDIKCFA YRLPHENGFV TAHANTLGSY FEVNKAIQKS FTRLMEYRGN GKNFNYKTDK 

       310        320        330        340        350        360 
IAAHESRIEE SAEIDKDSVI KRSFISDNCR IGEKTKLKES IIAKGVVIGN GASISNSIIC 

       370        380        390        400 
DGVEIGENAD VTNCIVAKDQ KVPAKGKVQN EVVEDGEDEE WTDD 

References and documentation are available.

Number of amino acids: 404

Molecular weight: 44778.57

Theoretical pI: 6.28

Amino acid composition: 
Ala (A)  27	  6.7%
Arg (R)  12	  3.0%
Asn (N)  18	  4.5%
Asp (D)  29	  7.2%
Cys (C)  11	  2.7%
Gln (Q)   9	  2.2%
Glu (E)  26	  6.4%
Gly (G)  22	  5.4%
His (H)  11	  2.7%
Ile (I)  33	  8.2%
Leu (L)  35	  8.7%
Lys (K)  39	  9.7%
Met (M)   7	  1.7%
Phe (F)  18	  4.5%
Pro (P)  15	  3.7%
Ser (S)  31	  7.7%
Thr (T)  20	  5.0%
Trp (W)   1	  0.2%
Tyr (Y)   9	  2.2%
Val (V)  31	  7.7%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 55
Total number of positively charged residues (Arg + Lys): 51

Atomic composition:

Carbon      C	      1993
Hydrogen    H	      3197
Nitrogen    N	       529
Oxygen      O	       602
Sulfur      S	        18

Formula: C1993H3197N529O602S18
Total number of atoms: 6339

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    19535
Abs 0.1% (=1 g/l)   0.436, assuming all pairs of Cys residues form cystines


Ext. coefficient    18910
Abs 0.1% (=1 g/l)   0.422, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 26.58
This classifies the protein as stable.



Aliphatic index: 94.58

Grand average of hydropathicity (GRAVY): -0.151