ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MHERLNFPKV ELHLHLDGAV RFDTLIDLSQ QKGIPLAGAK TVEELKKVLV THEPANLSKV 

        70         80         90        100        110        120 
LEAFEIFLPV IRGDLAAIER VAYELCEDQH NNGVVYFEGR YSPHLLLCND YPEVTAAHVV 

       130        140        150        160        170        180 
AAVKKGFDRG EKQFGIKARS ILCCIRGLDK KFPQLILDLA TDLKQLGVVA IDVAGSAHGA 

       190        200        210        220        230        240 
DEQYEPEVVA AFQEAHKRGI HRTVHAGESG GPKEVIKAIE DMYAERIGHG YRVMRDEEMY 

       250        260        270        280        290        300 
LEHFVNSKNV HLEACPYSSV MTGAVPLDWK NHPIARWAKD DVNFSVSRDD PTCFDNSMLS 

       310        320        330        340 
ELTLVHKQIG LDVHQLWKAQ LNAARSCFLP EDEKAELVKR VEAGEPPRP 

References and documentation are available.

Number of amino acids: 349

Molecular weight: 39081.69

Theoretical pI: 5.79

Amino acid composition: 
Ala (A)  33	  9.5%
Arg (R)  18	  5.2%
Asn (N)  11	  3.2%
Asp (D)  22	  6.3%
Cys (C)   7	  2.0%
Gln (Q)  11	  3.2%
Glu (E)  31	  8.9%
Gly (G)  22	  6.3%
His (H)  17	  4.9%
Ile (I)  16	  4.6%
Leu (L)  35	 10.0%
Lys (K)  23	  6.6%
Met (M)   6	  1.7%
Phe (F)  13	  3.7%
Pro (P)  17	  4.9%
Ser (S)  14	  4.0%
Thr (T)   9	  2.6%
Trp (W)   3	  0.9%
Tyr (Y)   9	  2.6%
Val (V)  32	  9.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 53
Total number of positively charged residues (Arg + Lys): 41

Atomic composition:

Carbon      C	      1744
Hydrogen    H	      2743
Nitrogen    N	       485
Oxygen      O	       510
Sulfur      S	        13

Formula: C1744H2743N485O510S13
Total number of atoms: 5495

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    30285
Abs 0.1% (=1 g/l)   0.775, assuming all pairs of Cys residues form cystines


Ext. coefficient    29910
Abs 0.1% (=1 g/l)   0.765, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 37.53
This classifies the protein as stable.



Aliphatic index: 93.04

Grand average of hydropathicity (GRAVY): -0.262