ProtParam
User-provided sequence:
10 20 30 40 50 60
MHFRDFNYNF SSLIACVANG DVFSESETRA KFESLFRTYD KDITFQYFKS FKRVRINFSN
70 80 90 100 110 120
PLSAADARLQ LHKTEFLGKE MKLYFAQTLH IGSSHLAPPN PDKQFLISPP ASPPVGWKQV
130 140 150 160 170 180
EDATPVINYD LLYAISKLGP GEKYELHAAT DTTPSVVVHV CESDQEEEEE EEEEMERMKR
190
PKPKIIQTRR PEYTPIHLS
References and
documentation are available.
Number of amino acids: 199
Molecular weight: 22947.92
Theoretical pI: 5.67
Total number of negatively charged residues (Asp + Glu): 30
Total number of positively charged residues (Arg + Lys): 24
Atomic composition:
Carbon C 1033
Hydrogen H 1582
Nitrogen N 272
Oxygen O 309
Sulfur S 6
Formula: C
1033H
1582N
272O
309S
6
Total number of atoms: 3202
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 17545
Abs 0.1% (=1 g/l) 0.765, assuming all pairs of Cys residues form cystines
Ext. coefficient 17420
Abs 0.1% (=1 g/l) 0.759, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 59.20
This classifies the protein as unstable.
Aliphatic index: 70.10
Grand average of hydropathicity (GRAVY): -0.592