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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MHFRDFNYNF SSLIACVANG DVFSESETRA KFESLFRTYD KDITFQYFKS FKRVRINFSN

70 80 90 100 110 120
PLSAADARLQ LHKTEFLGKE MKLYFAQTLH IGSSHLAPPN PDKQFLISPP ASPPVGWKQV

130 140 150 160 170 180
EDATPVINYD LLYAISKLGP GEKYELHAAT DTTPSVVVHV CESDQEEEEE EEEEMERMKR

190
PKPKIIQTRR PEYTPIHLS


References and documentation are available.

Number of amino acids: 199 Molecular weight: 22947.92 Theoretical pI: 5.67
Amino acid composition: 
Ala (A) 13 6.5% Arg (R) 10 5.0% Asn (N) 7 3.5% Asp (D) 10 5.0% Cys (C) 2 1.0% Gln (Q) 7 3.5% Glu (E) 20 10.1% Gly (G) 6 3.0% His (H) 7 3.5% Ile (I) 10 5.0% Leu (L) 15 7.5% Lys (K) 14 7.0% Met (M) 4 2.0% Phe (F) 13 6.5% Pro (P) 15 7.5% Ser (S) 16 8.0% Thr (T) 11 5.5% Trp (W) 1 0.5% Tyr (Y) 8 4.0% Val (V) 10 5.0% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 30 Total number of positively charged residues (Arg + Lys): 24 Atomic composition: Carbon C 1033 Hydrogen H 1582 Nitrogen N 272 Oxygen O 309 Sulfur S 6 Formula: C1033H1582N272O309S6 Total number of atoms: 3202 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 17545 Abs 0.1% (=1 g/l) 0.765, assuming all pairs of Cys residues form cystines Ext. coefficient 17420 Abs 0.1% (=1 g/l) 0.759, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 59.20 This classifies the protein as unstable. Aliphatic index: 70.10 Grand average of hydropathicity (GRAVY): -0.592