ProtParam
User-provided sequence:
10 20 30 40 50 60
MHGPTSKAIS RNVRSVKRPR RAPRPVVSTQ AMNKLSNVTL SAEQEKLRER VLSFMRSNLS
70 80 90 100 110 120
QYKSDWKHPA MFVIQGDAGT GKSVILNSLF NEIQKLSQFS PSSEDILHGT HNYLVVNHPE
130 140 150 160 170 180
MLKLYIRISD SFKYISKSSL ERPTSLINNL QKRKVMADVV IVDEAHLLAT SKDAFKRFYG
190 200 210 220 230 240
ENHLKDLMSL CKVLVLVYDD KQALRMGSYW DEGSNNGATL KDFYNEIPPK SRDWYTLKQQ
250 260 270 280 290 300
FRVAAPQNVL NWIDQISVAG KIPPIESVLS KGNADCADDK IKNFDFKIWD DCGAMYEAIK
310 320 330 340 350 360
EKDRQYGQCR MLSTYDFPYR LDGKDYYVEC GDNFKVRWDR YTPREVTPWS ERCDTIDEVG
370 380 390 400 410 420
SVYTIQGFDL NYAGVILGRS IGYDAANDCI KLRPELYDDR AGFTKKKNIH NAEDVKQKII
430 440 450
MNSINVLLTR GVRGLYVYAY DPELRERLLR PSKK
References and
documentation are available.
Number of amino acids: 454
Molecular weight: 52256.69
Theoretical pI: 9.11
Total number of negatively charged residues (Asp + Glu): 57
Total number of positively charged residues (Arg + Lys): 69
Atomic composition:
Carbon C 2326
Hydrogen H 3663
Nitrogen N 649
Oxygen O 685
Sulfur S 18
Formula: C
2326H
3663N
649O
685S
18
Total number of atoms: 7341
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 73145
Abs 0.1% (=1 g/l) 1.400, assuming all pairs of Cys residues form cystines
Ext. coefficient 72770
Abs 0.1% (=1 g/l) 1.393, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 36.56
This classifies the protein as stable.
Aliphatic index: 82.00
Grand average of hydropathicity (GRAVY): -0.544