ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MHHHHHPCNR KPFTTIFSFF LLYLNLHNQQ IIEARNPSQF TTNPSPDVSI PEIKRHLQQY 

        70         80         90        100        110        120 
GYLPQNKESD DVSFEQALVR YQKNLGLPIT GKPDSDTLSQ ILLPRCGFPD DVEPKTAPFH 

       130        140        150        160        170        180 
TGKKYVYFPG RPRWTRDVPL KLTYAFSQEN LTPYLAPTDI RRVFRRAFGK WASVIPVSFI 

       190        200        210        220        230        240 
ETEDYVIADI KIGFFNGDHG DGEPFDGVLG VLAHTFSPEN GRLHLDKAET WAVDFDEEKS 

       250        260        270        280        290        300 
SVAVDLESVA VHEIGHVLGL GHSSVKDAAM YPTLKPRSKK VNLNMDDVVG VQSLYGTNPN 

       310        320        330        340 
FTLNSLLASE TSTNLADGSR IRSQGMIYST LSTVIALCFL NW 

References and documentation are available.

Number of amino acids: 342

Molecular weight: 38505.57

Theoretical pI: 6.26

Amino acid composition: 
Ala (A)  18	  5.3%
Arg (R)  16	  4.7%
Asn (N)  17	  5.0%
Asp (D)  22	  6.4%
Cys (C)   3	  0.9%
Gln (Q)  12	  3.5%
Glu (E)  16	  4.7%
Gly (G)  21	  6.1%
His (H)  14	  4.1%
Ile (I)  17	  5.0%
Leu (L)  33	  9.6%
Lys (K)  17	  5.0%
Met (M)   4	  1.2%
Phe (F)  20	  5.8%
Pro (P)  24	  7.0%
Ser (S)  26	  7.6%
Thr (T)  22	  6.4%
Trp (W)   4	  1.2%
Tyr (Y)  12	  3.5%
Val (V)  24	  7.0%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 38
Total number of positively charged residues (Arg + Lys): 33

Atomic composition:

Carbon      C	      1741
Hydrogen    H	      2666
Nitrogen    N	       468
Oxygen      O	       508
Sulfur      S	         7

Formula: C1741H2666N468O508S7
Total number of atoms: 5390

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    40005
Abs 0.1% (=1 g/l)   1.039, assuming all pairs of Cys residues form cystines


Ext. coefficient    39880
Abs 0.1% (=1 g/l)   1.036, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 39.77
This classifies the protein as stable.



Aliphatic index: 82.63

Grand average of hydropathicity (GRAVY): -0.332