ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MHHQTKLDVF IIRAYNLLSN ESVISGASLQ SVTNSPQTTT NTPSGMVNGA VGTGIANPTG 

        70         80         90        100        110        120 
LMGSDSTPNI DEIITSTGSN ALTKTNSDSA NGTPNGNSSS TSAISNASNP ATTGNNASSS 

       130        140        150        160        170        180 
ATSNGIYTQA QYSQLFAKIS KLYNATLSSG SIDDRSTSPK SAIELYQRFQ QMIKELELSF 

       190        200        210        220        230        240 
DASPYAKYFR RLDGRLWQIK TDSELENDEL WRLVSMSIFT VFDPQTGQIL TQGRRKGNSL 

       250        260        270        280        290        300 
NTSTKGSPSD LQGINNGNNN GNNGNIGNGS NIKNYGNKNM PNNRTKKRGT RVAKNAKNGK 

       310        320        330        340        350        360 
NNKNSNKERN GITDTSAFSN TTISNPGTNM LFDPSLSQQL QKRLQTLSQD VNSRSLTGYY 

       370        380        390        400        410        420 
TQPTSPGSGG FEFGLSHADL NPNASSNTMG YNTMSNNGSH SWKRRSLGSL DVNTLDDEAV 

       430        440        450        460        470        480 
EELLQLTNTS KRQRPMTTAA EGALINDGPD TNLNANNTQM KVDLNPSNSM GPIDTEAVIR 

       490        500        510        520        530 
PLKEAYDAII SEKGQRIVQL ERELELQRQE TQWLRKMLIE DMGCVRSMLR DLQR 

References and documentation are available.

Number of amino acids: 534

Molecular weight: 58062.03

Theoretical pI: 9.00

Amino acid composition: 
Ala (A)  30	  5.6%
Arg (R)  27	  5.1%
Asn (N)  62	 11.6%
Asp (D)  26	  4.9%
Cys (C)   1	  0.2%
Gln (Q)  29	  5.4%
Glu (E)  22	  4.1%
Gly (G)  42	  7.9%
His (H)   4	  0.7%
Ile (I)  28	  5.2%
Leu (L)  45	  8.4%
Lys (K)  26	  4.9%
Met (M)  15	  2.8%
Phe (F)  11	  2.1%
Pro (P)  21	  3.9%
Ser (S)  65	 12.2%
Thr (T)  49	  9.2%
Trp (W)   4	  0.7%
Tyr (Y)  12	  2.2%
Val (V)  15	  2.8%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 48
Total number of positively charged residues (Arg + Lys): 53

Atomic composition:

Carbon      C	      2461
Hydrogen    H	      3970
Nitrogen    N	       744
Oxygen      O	       848
Sulfur      S	        16

Formula: C2461H3970N744O848S16
Total number of atoms: 8039

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    39880
Abs 0.1% (=1 g/l)   0.687, assuming all pairs of Cys residues form cystines


Ext. coefficient    39880
Abs 0.1% (=1 g/l)   0.687, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 39.06
This classifies the protein as stable.



Aliphatic index: 67.08

Grand average of hydropathicity (GRAVY): -0.753