ProtParam
User-provided sequence:
10 20 30 40 50 60
MHILFLFIFH CLAFKDLIFF KQYVPFAAAG GYPISFLFIK VLTASTNLLL SSSSGGSWNK
70 80 90 100 110
LSKESQLLKV ILTHFLVPIF FFLFQYIILS EDRQQERQPK FRDNAKFDGH AKTCHI
References and
documentation are available.
Number of amino acids: 116
Molecular weight: 13437.84
Theoretical pI: 9.43
Total number of negatively charged residues (Asp + Glu): 7
Total number of positively charged residues (Arg + Lys): 12
Atomic composition:
Carbon C 641
Hydrogen H 966
Nitrogen N 154
Oxygen O 157
Sulfur S 3
Formula: C
641H
966N
154O
157S
3
Total number of atoms: 1921
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 10095
Abs 0.1% (=1 g/l) 0.751, assuming all pairs of Cys residues form cystines
Ext. coefficient 9970
Abs 0.1% (=1 g/l) 0.742, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 55.06
This classifies the protein as unstable.
Aliphatic index: 103.45
Grand average of hydropathicity (GRAVY): 0.365