ProtParam
User-provided sequence:
10 20 30 40 50 60
MHISLPTRNK SYFRIRTRTY QIGLYHSDSS PIRDISVLHL LIATLCTIFF PIFFSLSKVQ
70 80 90 100 110 120
VVQWQGTTIS KNCIALTMSF PLNAIPGMYL IIAFPRLQTV IPLQRNTPVR ITKSVIVKGA
130
VSVPRISSPM H
References and
documentation are available.
Number of amino acids: 131
Molecular weight: 14827.68
Theoretical pI: 11.01
Total number of negatively charged residues (Asp + Glu): 2
Total number of positively charged residues (Arg + Lys): 14
Atomic composition:
Carbon C 681
Hydrogen H 1098
Nitrogen N 182
Oxygen O 175
Sulfur S 6
Formula: C
681H
1098N
182O
175S
6
Total number of atoms: 2142
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 11585
Abs 0.1% (=1 g/l) 0.781, assuming all pairs of Cys residues form cystines
Ext. coefficient 11460
Abs 0.1% (=1 g/l) 0.773, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 52.15
This classifies the protein as unstable.
Aliphatic index: 112.29
Grand average of hydropathicity (GRAVY): 0.366