ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MHKGKKNYPN LITSFRMNLK KIILNHDRFS HPERWKTNAL LRFTFVYIKF LFDLMIIKNP 

        70         80         90        100        110        120 
LRMVGKTYRD AVTALNSLQS NYANIMAIRQ TGDRKNTMTL LEMHEWSRRI GYSASDFNKL 

       130        140        150        160        170        180 
NIVHITGTKG KGSTAAFTSS ILGQYKEQLP RIGLYTSPHL KSVRERIRIN GEPISEEKFA 

       190        200        210        220        230        240 
KYFFEVWDRL DSTTSSLDKF PHMIPGSKPG YFKFLTLLSF HTFIQEDCKS CVYEVGVGGE 

       250        260        270        280        290        300 
LDSTNIIEKP IVCGVTLLGI DHTFMLGDTI EEIAWNKGGI FKSGAPAFTV EKQPPQGLTI 

       310        320        330        340        350        360 
LKERAEERKT TLTEVPPFKQ LENVKLGIAG EFQKSNASLA VMLASEILHT SNILEEKIKC 

       370        380        390        400        410        420 
SSNASIPEKF IIGLQNTKWE GRCQVLEKGK NVWYIDGAHT KDSMVAASTW FRDMVRLSKR 

       430        440        450        460        470        480 
KKILLFNQQS RDANALVNNL YSSVSPEITF DDVIFTTNVT WKSGSYSADL VSMNTSQEDV 

       490        500        510        520        530        540 
EKLKVQESLV KNWNKIDDNR AKTHVTASIE EANELIETLY DEPADIFVTG SLHLVGGLLV 


VFDRIDVK 

References and documentation are available.

Number of amino acids: 548

Molecular weight: 62151.44

Theoretical pI: 8.92

Amino acid composition: 
Ala (A)  28	  5.1%
Arg (R)  24	  4.4%
Asn (N)  31	  5.7%
Asp (D)  26	  4.7%
Cys (C)   5	  0.9%
Gln (Q)  15	  2.7%
Glu (E)  37	  6.8%
Gly (G)  32	  5.8%
His (H)  13	  2.4%
Ile (I)  42	  7.7%
Leu (L)  51	  9.3%
Lys (K)  47	  8.6%
Met (M)  13	  2.4%
Phe (F)  28	  5.1%
Pro (P)  18	  3.3%
Ser (S)  43	  7.8%
Thr (T)  39	  7.1%
Trp (W)   9	  1.6%
Tyr (Y)  14	  2.6%
Val (V)  33	  6.0%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 63
Total number of positively charged residues (Arg + Lys): 71

Atomic composition:

Carbon      C	      2795
Hydrogen    H	      4420
Nitrogen    N	       748
Oxygen      O	       817
Sulfur      S	        18

Formula: C2795H4420N748O817S18
Total number of atoms: 8798

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    70610
Abs 0.1% (=1 g/l)   1.136, assuming all pairs of Cys residues form cystines


Ext. coefficient    70360
Abs 0.1% (=1 g/l)   1.132, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 38.20
This classifies the protein as stable.



Aliphatic index: 88.76

Grand average of hydropathicity (GRAVY): -0.286