ProtParam
User-provided sequence:
10 20 30 40 50 60
MHLKKGKRSI STVWRLLWKR FYSVNSKTNM HFSRSRKKPV TNFTRTNGLL LSCNGDTFPY
70 80 90 100 110 120
LRTLWRYFNA PGNLMFVTTN IVAFMGIVTY NTLVTISSER AFEEQMMAAQ VSLAKQREEL
130 140 150 160 170 180
ETTALSLPRD IELRGEEDDI KWEQPDVAHV REDPLVEEQN AKLDTPIKQY TLGDLILNKR
190 200 210 220 230 240
ENVTDYDSQR AKASIFHMLY AYMLYRDVIQ PTTMTQNNNS EEWRREVELL TKGKEVQGTH
250 260 270 280 290 300
RRIDVFYDLW NKNFDKIVTS PEKVQNFQLP NWSKYPTILK FICTELHDNS LKTLGEFKQF
310 320 330 340 350 360
YGKVRSNEVK KLLGLWLYDH SFLFPHNIYD NRTEEDFYDI LINDSMQDNR IFQKYSSIVM
370 380 390 400 410 420
NPYNERTQLF FPNVNSPSVN KPVPSISLET YTRLLKGYIN LQETGCKYDY NDNIFKLISI
430 440 450 460 470 480
LKLNCFLQRN KKKHAGPTVR ILLPRDEDRS QILGTISQAE KRTCYQILSK NRDVVALLKR
ISDIQADSS
References and
documentation are available.
Number of amino acids: 489
Molecular weight: 57487.69
Theoretical pI: 9.14
Total number of negatively charged residues (Asp + Glu): 58
Total number of positively charged residues (Arg + Lys): 69
Atomic composition:
Carbon C 2578
Hydrogen H 4042
Nitrogen N 706
Oxygen O 753
Sulfur S 16
Formula: C
2578H
4042N
706O
753S
16
Total number of atoms: 8095
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 77030
Abs 0.1% (=1 g/l) 1.340, assuming all pairs of Cys residues form cystines
Ext. coefficient 76780
Abs 0.1% (=1 g/l) 1.336, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 51.40
This classifies the protein as unstable.
Aliphatic index: 83.89
Grand average of hydropathicity (GRAVY): -0.567