ProtParam
User-provided sequence:
10 20 30 40 50
MHLMYTLGPD GKRIYTLKKV TESGEITKSA HPARFSPDDK YSRQRVTLKK RFGLVPGQ
References and
documentation are available.
Number of amino acids: 58
Molecular weight: 6635.71
Theoretical pI: 10.16
Total number of negatively charged residues (Asp + Glu): 5
Total number of positively charged residues (Arg + Lys): 12
Atomic composition:
Carbon C 296
Hydrogen H 480
Nitrogen N 86
Oxygen O 83
Sulfur S 2
Formula: C
296H
480N
86O
83S
2
Total number of atoms: 947
Extinction coefficients:
This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 4470
Abs 0.1% (=1 g/l) 0.674
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 46.81
This classifies the protein as unstable.
Aliphatic index: 65.52
Grand average of hydropathicity (GRAVY): -0.795