ProtParam
User-provided sequence:
10 20 30 40 50 60
MHNFSSTVTQ VLQKYRLNGV DIDWEFPVWS RDAQKSDKAH FATFLRILKS HLKPADLKLS
70 80 90 100 110 120
VAVSGPPTIS RKAYDVDALK MYADMVQIMN YDFHVFNRYS NPFVGFNAPL HPMRAEISVL
130 140 150 160 170 180
GEMNAEASMK TWYDLGLPRN ISFFGIPTYA RAFQLLTHYL HKPYSPAIRA RPEITNLPDV
190 200 210 220 230 240
CIFAQSGYYT TVWNHHAQAP YLYGDDGIWV SYENQQSILA KMAFARKLQV GGVMVFSIGS
250 260
DDVTGKCGHG PYPLLTKISK LANF
References and
documentation are available.
Number of amino acids: 264
Molecular weight: 29925.37
Theoretical pI: 9.04
Total number of negatively charged residues (Asp + Glu): 21
Total number of positively charged residues (Arg + Lys): 26
Atomic composition:
Carbon C 1370
Hydrogen H 2073
Nitrogen N 359
Oxygen O 375
Sulfur S 11
Formula: C
1370H
2073N
359O
375S
11
Total number of atoms: 4188
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 49975
Abs 0.1% (=1 g/l) 1.670, assuming all pairs of Cys residues form cystines
Ext. coefficient 49850
Abs 0.1% (=1 g/l) 1.666, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 33.63
This classifies the protein as stable.
Aliphatic index: 81.67
Grand average of hydropathicity (GRAVY): -0.159