ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MHQDFQEDEH EYPDIRRNPL HEVTMTSYIL GILLGIFVGL FPQIRFKNFN LFIIALSLFH 

        70         80         90        100        110        120 
FLEYYITAKY NPLKVHSESF LLNNGKSYMA AHSFAILECL VESFLFPDLK IFSYSLATKL 

       130        140        150        160        170        180 
CTVLGCLLVI LGQYTRTIAM HTAGHSFSHI VKTKKESDHV LVKTGVYSWS RHPSYLGFFW 

       190        200        210        220        230 
WAIGTQLLLL NPLSLVIFIF VLWKFFSDRI RVEEKYLIEF FSAEYIEYKN KVGVGIPFI 

References and documentation are available.

Number of amino acids: 239

Molecular weight: 27887.68

Theoretical pI: 7.77

Amino acid composition: 
Ala (A)  10	  4.2%
Arg (R)   7	  2.9%
Asn (N)   8	  3.3%
Asp (D)   6	  2.5%
Cys (C)   3	  1.3%
Gln (Q)   5	  2.1%
Glu (E)  14	  5.9%
Gly (G)  12	  5.0%
His (H)  11	  4.6%
Ile (I)  21	  8.8%
Leu (L)  32	 13.4%
Lys (K)  14	  5.9%
Met (M)   4	  1.7%
Phe (F)  23	  9.6%
Pro (P)   8	  3.3%
Ser (S)  18	  7.5%
Thr (T)  11	  4.6%
Trp (W)   4	  1.7%
Tyr (Y)  13	  5.4%
Val (V)  15	  6.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 20
Total number of positively charged residues (Arg + Lys): 21

Atomic composition:

Carbon      C	      1325
Hydrogen    H	      1989
Nitrogen    N	       313
Oxygen      O	       335
Sulfur      S	         7

Formula: C1325H1989N313O335S7
Total number of atoms: 3969

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    41495
Abs 0.1% (=1 g/l)   1.488, assuming all pairs of Cys residues form cystines


Ext. coefficient    41370
Abs 0.1% (=1 g/l)   1.483, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 35.73
This classifies the protein as stable.



Aliphatic index: 108.87

Grand average of hydropathicity (GRAVY): 0.333