ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MHRILNINQY HPQLGDDGMV APWILWRLWK NRNDLVFKGK QFDAPDMELG MQNAASVASA 

        70         80         90        100        110        120 
GCLETPTRTC FGWVQGLSMT QSALVTEAEA MRWAVLSLSR LNYRKVIFES DSQQLVSILV 

       130        140        150        160        170        180 
DNGDMPILDP ILQDIKLLLQ HFEETKFVFM HRGGNGVADR IAKESLSLEN YDPKLYSIVP 

       190 
YWVKSFVELD KL 

References and documentation are available.

Number of amino acids: 192

Molecular weight: 21947.32

Theoretical pI: 5.56

Amino acid composition: 
Ala (A)  12	  6.2%
Arg (R)   9	  4.7%
Asn (N)   9	  4.7%
Asp (D)  13	  6.8%
Cys (C)   2	  1.0%
Gln (Q)  10	  5.2%
Glu (E)  10	  5.2%
Gly (G)  11	  5.7%
His (H)   4	  2.1%
Ile (I)  10	  5.2%
Leu (L)  24	 12.5%
Lys (K)  10	  5.2%
Met (M)   8	  4.2%
Phe (F)   8	  4.2%
Pro (P)   8	  4.2%
Ser (S)  13	  6.8%
Thr (T)   6	  3.1%
Trp (W)   6	  3.1%
Tyr (Y)   5	  2.6%
Val (V)  14	  7.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 23
Total number of positively charged residues (Arg + Lys): 19

Atomic composition:

Carbon      C	       990
Hydrogen    H	      1542
Nitrogen    N	       262
Oxygen      O	       282
Sulfur      S	        10

Formula: C990H1542N262O282S10
Total number of atoms: 3086

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    40575
Abs 0.1% (=1 g/l)   1.849, assuming all pairs of Cys residues form cystines


Ext. coefficient    40450
Abs 0.1% (=1 g/l)   1.843, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 40.81
This classifies the protein as unstable.



Aliphatic index: 96.46

Grand average of hydropathicity (GRAVY): -0.124