ProtParam
User-provided sequence:
10 20 30 40 50 60
MHRKSLRRAS ATVPSAPYRK QIISNAHNKP SLFSKIKTFF TQKDSARVSP RNNVANKQPR
70 80 90 100 110 120
NESFNRRISS MPGGYFHSEI SPDSTVNRSV VVSAVGEARN DIENKEEEYD ETHETNISNA
130 140 150 160 170 180
KLANFFSKKG NEPLSEIEIE GVMSLLQKSS KSMITSEGEQ KSAEGNNIDQ SLILKESGST
190 200 210 220 230 240
PISISNAPTF NPKYDTSNAS MNTTLGSIGS RKYSFNYSSL PSPYKTTVYR YSAAKKIPDT
250 260 270 280 290 300
YTANTSAQSI ASAKSVRSGV SKSAPSKKIS NTAAALVSLL DENDSKKNNA ASELANPYSS
310 320 330 340 350 360
YVSQIRKHKR VSPNAAPRQE ISEEETTVKP LFQNVPEQGE EPMKQLNATK ISPSAPSKDS
370 380 390 400 410 420
FTKYKPARSS SLRSNVVVAE TSPEKKDGGD KPPSSAFNFS FNTSRNVEPT ENAYKSENAP
430 440 450 460 470 480
SASSKEFNFT NLQAKPLVGK PKTELTKGDS TPVQPDLSVT PQKSSSKGFV FNSVQKKSRS
490 500 510 520 530
NLSQENDNEG KHISASIDND FSEEKAEEFD FNVPVVSKQL GNGLVDENKV EAFKSLYTF
References and
documentation are available.
Number of amino acids: 539
Molecular weight: 59039.35
Theoretical pI: 9.34
Total number of negatively charged residues (Asp + Glu): 60
Total number of positively charged residues (Arg + Lys): 73
Atomic composition:
Carbon C 2564
Hydrogen H 4082
Nitrogen N 730
Oxygen O 857
Sulfur S 6
Formula: C
2564H
4082N
730O
857S
6
Total number of atoms: 8239
Extinction coefficients:
This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 22350
Abs 0.1% (=1 g/l) 0.379
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 58.94
This classifies the protein as unstable.
Aliphatic index: 58.83
Grand average of hydropathicity (GRAVY): -0.833