ProtParam
User-provided sequence:
10 20 30 40 50 60
MHSGKRPLSP ESMAGNREEK KELCCCSTLS ESDVSDFVSE LTGQPIPSSI DDQSSSLTLQ
70 80 90 100 110 120
EKSNSRQRNY RGVRQRPWGK WAAEIRDPNK AARVWLGTFD TAEEAALAYD KAAFEFRGHK
130 140 150 160 170 180
AKLNFPEHIR VNPTQLYPSP ATSHDRIIVT PPSPPPPIAP DILLDQYGHF QSRSSDSSAN
190 200 210
LSMNMLSSSS SSLNHQGLRP NLEDGENVKN ISIHKRRK
References and
documentation are available.
Number of amino acids: 218
Molecular weight: 24297.08
Theoretical pI: 8.43
Total number of negatively charged residues (Asp + Glu): 26
Total number of positively charged residues (Arg + Lys): 28
Atomic composition:
Carbon C 1050
Hydrogen H 1662
Nitrogen N 316
Oxygen O 335
Sulfur S 7
Formula: C
1050H
1662N
316O
335S
7
Total number of atoms: 3370
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 22585
Abs 0.1% (=1 g/l) 0.930, assuming all pairs of Cys residues form cystines
Ext. coefficient 22460
Abs 0.1% (=1 g/l) 0.924, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 70.87
This classifies the protein as unstable.
Aliphatic index: 64.50
Grand average of hydropathicity (GRAVY): -0.821