ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MHSNVFVGVK PGFNHKQHSK KSRFLENVSS HSPELPSNYD YVLLPITTPR YKEIVGQVFK 

        70         80         90        100        110        120 
DFQRQSIQNW KPLQIPEPQL QDICIPPFNV KKLDNDDTPS YIGLLSSWLE LESRDPNVRD 

       130        140        150        160        170        180 
LGLKVLLNEC KYARFVGINK LILAPPRDLS NLQLYGQMIY RLLQNRIVFA APALTISISL 

       190        200        210        220        230        240 
PLYEDSDPLA TWELWNTVRK QCEYHPSLTI SLALPRTRTP SYVLNRWLAE PVSCLLVSSS 

       250        260        270        280        290        300 
IFASNQYDYP VLHKFNQNLI LKFQKVNGDS QILGNELCVI LHGMEKYANN VKGGESAYLE 

       310        320        330        340        350        360 
YINYLLKKGD KVLNSNSNHQ FLLQEDSRIM PPLKPHSDNL LNSTYLTFEK DLVKYDLYES 

       370        380        390        400        410        420 
AILEALQDLA PRASAKRPLV ILVAGAGRGP LVDRTFKIIS MLFMDSKVSI IAIEKNPQAY 

       430        440        450        460        470        480 
LYLQKRNFDC WDNRVKLIKE DMTKWQINEP SEKRIQIDLC ISELLGSFGC NELSPECLWS 

       490        500        510        520        530        540 
IEKYHSHNDT IFIPRSYSSY IAPISSPLFY QKLSQTNRSL EAPWIVHRVP YCILSSRVNE 

       550        560        570        580        590        600 
VWRFEHPMAQ KDTVQDEDDF TVEFSQSSLN EFKIKHRGEI HGFIGFFSAN LYNNIFLSTL 

       610        620        630        640        650        660 
PNDSTVRLKF SEETLMNTRR EENLIKKCDH TPNMTSWSPI IFPLKQPISF IDDSELSVLM 

       670        680        690        700        710        720 
SRIHSDTEQK VWYEWSLESF IYLMLSNYTS AVTAASMTIP RSIVTDDTKT LAHNRHYSAT 

       730        740        750        760        770        780 
TNQKLDNQID LDQDIENEEE QGFLSNLETG WQSVQDIHGL SETAKPDHLD SINKPMFDLK 

       790        800        810        820 
STKALEPSNE LPRHEDLEED VPEVHVRVKT SVSTLHNVCG RAFSLPL 

References and documentation are available.

Number of amino acids: 827

Molecular weight: 95153.44

Theoretical pI: 5.93

Amino acid composition: 
Ala (A)  32	  3.9%
Arg (R)  36	  4.4%
Asn (N)  52	  6.3%
Asp (D)  47	  5.7%
Cys (C)  12	  1.5%
Gln (Q)  36	  4.4%
Glu (E)  54	  6.5%
Gly (G)  25	  3.0%
His (H)  24	  2.9%
Ile (I)  56	  6.8%
Leu (L)  98	 11.9%
Lys (K)  50	  6.0%
Met (M)  14	  1.7%
Phe (F)  34	  4.1%
Pro (P)  49	  5.9%
Ser (S)  81	  9.8%
Thr (T)  37	  4.5%
Trp (W)  14	  1.7%
Tyr (Y)  31	  3.7%
Val (V)  45	  5.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 101
Total number of positively charged residues (Arg + Lys): 86

Atomic composition:

Carbon      C	      4282
Hydrogen    H	      6667
Nitrogen    N	      1135
Oxygen      O	      1267
Sulfur      S	        26

Formula: C4282H6667N1135O1267S26
Total number of atoms: 13377

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   123940
Abs 0.1% (=1 g/l)   1.303, assuming all pairs of Cys residues form cystines


Ext. coefficient   123190
Abs 0.1% (=1 g/l)   1.295, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 48.38
This classifies the protein as unstable.



Aliphatic index: 92.27

Grand average of hydropathicity (GRAVY): -0.368