ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MHSSLEPEKM EEGGGSNSLK RKFSEIDGDQ NLDSVSSPMM TDSNGSYELK VYEVAKNRNI 

        70         80         90        100        110        120 
IAVLGTGIDK SEITKRLIKA MGSSDTDKRL IIFLAPTVNL QCCEIRALVN LKVEEYFGAK 

       130        140        150        160        170        180 
GVDKWTSQRW DEEFSKHDVL VMTPQILLDV LRSAFLKLEM VCLLIIDECH HTTGNHPYAK 

       190        200        210        220        230        240 
LMKIFNPEER EGVEKFATTV KEGPILYNPS PSCSLELKEK LETSHLKFDA SLRRLQELGK 

       250        260        270        280        290        300 
DSFLNMDNKF ETYQKRLSID YREILHCLDN LGLICAHLAA EVCLEKISDT KEESETYKEC 

       310        320        330        340        350        360 
SMVCKEFLED ILSTIGVYLP QDDKSLVDLQ QNHLSAVISG HVSPKLKELF HLLDSFRGDK 

       370        380        390        400        410        420 
QKQCLILVER IITAKVIERF VKKEASLAYL NVLYLTENNP STNVSAQKMQ IEIPDLFQHG 

       430        440        450        460        470        480 
KVNLLFITDV VEEGFQVPDC SCMVCFDLPK TMCSYSQSQK HAKQSNSKSI MFLERGNPKQ 

       490        500        510        520        530        540 
RDHLHDLMRR EVLIQDPEAP NLKSCPPPVK NGHGVKEIGS MVIPDSNITV SEEAASTQTM 

       550        560        570        580        590        600 
SDPPSRNEQL PPCKKLRLDN NLLQSNGKEK VASSKSKSSS SAAGSKKRKE LHGTTCANAL 

       610        620        630        640        650        660 
SGTWGENIDG ATFQAYKFDF CCNISGEVYS SFSLLLESTL AEDVGKVEMD LYLVRKLVKA 

       670        680        690        700        710        720 
SVSPCGQIRL SQEELVKAKY FQQFFFNGMF GKLFVGSKSQ GTKREFLLQT DTSSLWHPAF 

       730        740        750        760        770        780 
MFLLLPVETN DLASSATIDW SAINSCASIV EFLKKNSLLD LRDSDGNQCN TSSGQEVLLD 

       790        800        810        820        830        840 
DKMEETNLIH FANASSDKNS LEELVVIAIH TGRIYSIVEA VSDSSAMSPF EVDASSGYAT 

       850        860        870        880        890        900 
YAEYFNKKYG IVLAHPNQPL MKLKQSHHAH NLLVDFNEEM VVKTEPKAGN VRKRKPNIHA 

       910        920        930        940        950        960 
HLPPELLARI DVPRAVLKSI YLLPSVMHRL ESLMLASQLR EEIDCSIDNF SISSTSILEA 

       970        980        990       1000       1010       1020 
VTTLTCPESF SMERLELLGD SVLKYVASCH LFLKYPDKDE GQLSRQRQSI ISNSNLHRLT 

      1030       1040       1050       1060       1070       1080 
TSRKLQGYIR NGAFEPRRWT APGQFSLFPV PCKCGIDTRE VPLDPKFFTE NMTIKIGKSC 

      1090       1100       1110       1120       1130       1140 
DMGHRWVVSK SVSDCAEALI GAYYVSGGLS ASLHMMKWLG IDVDFDPNLV VEAINRVSLR 

      1150       1160       1170       1180       1190       1200 
CYIPKEDELI ELERKIQHEF SAKFLLKEAI THSSLRESYS YERLEFLGDS VLDFLITRHL 

      1210       1220       1230       1240       1250       1260 
FNTYEQTGPG EMTDLRSACV NNENFAQVAV KNNLHTHLQR CATVLETQIN DYLMSFQKPD 

      1270       1280       1290       1300       1310       1320 
ETGRSIPSIQ GPKALGDVVE SIAGALLIDT RLDLDQVWRV FEPLLSPLVT PDKLQLPPYR 

      1330       1340       1350       1360       1370       1380 
ELNELCDSLG YFFRVKCSND GVKAQATIQL QLDDVLLTGD GSEQTNKLAL GKAASHLLTQ 

      1390       1400       1410       1420       1430       1440 
LEKRNISRKT SLGDNQSSMD VNLACNHSDR ETLTSETTEI QSIVIPFIGP INMKKGGPRG 

      1450       1460       1470       1480       1490       1500 
TLHEFCKKHL WPMPTFDTSE EKSRTPFEFI DGGEKRTSFS SFTSTITLRI PNREAVMYAG 

      1510       1520       1530 
EARPDKKSSF DSAVVELLYE LERRKIVIIQ K 

References and documentation are available.

Number of amino acids: 1531

Molecular weight: 172013.64

Theoretical pI: 5.90

Amino acid composition: 
Ala (A)  80	  5.2%
Arg (R)  67	  4.4%
Asn (N)  69	  4.5%
Asp (D)  86	  5.6%
Cys (C)  37	  2.4%
Gln (Q)  57	  3.7%
Glu (E) 119	  7.8%
Gly (G)  75	  4.9%
His (H)  40	  2.6%
Ile (I)  84	  5.5%
Leu (L) 175	 11.4%
Lys (K) 111	  7.3%
Met (M)  37	  2.4%
Phe (F)  64	  4.2%
Pro (P)  65	  4.2%
Ser (S) 150	  9.8%
Thr (T)  77	  5.0%
Trp (W)  10	  0.7%
Tyr (Y)  36	  2.4%
Val (V)  92	  6.0%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 205
Total number of positively charged residues (Arg + Lys): 178

Atomic composition:

Carbon      C	      7601
Hydrogen    H	     12115
Nitrogen    N	      2059
Oxygen      O	      2331
Sulfur      S	        74

Formula: C7601H12115N2059O2331S74
Total number of atoms: 24180

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   110890
Abs 0.1% (=1 g/l)   0.645, assuming all pairs of Cys residues form cystines


Ext. coefficient   108640
Abs 0.1% (=1 g/l)   0.632, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 43.50
This classifies the protein as unstable.



Aliphatic index: 88.63

Grand average of hydropathicity (GRAVY): -0.306