ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MHSTGEPQRG PAGPYHHPMP YHVDQIPTTS HPSWHQAPHP GLPTPPYHPQ QHPNYISMQP 

        70         80         90        100        110        120 
PPSQQQQMTP QALSTQQQQQ VQQQQQRQLY SSPSPSRGPA APAQNQKFRT EQWINNQAWP 

       130        140        150        160        170        180 
YSQSPAPPVA PSVMSYHQGD DRMSMLSVNT TMTNQFPDSQ RCYSSNGSTC ENVNPEMMQM 

       190        200        210        220        230 
NITQAAEQAI RFNFSYGLIH QIPCKDFIWQ KQYELGYDKI NLHKLIRQIC RTAYNKSLT 

References and documentation are available.

Number of amino acids: 239

Molecular weight: 27362.54

Theoretical pI: 8.66

Amino acid composition: 
Ala (A)  13	  5.4%
Arg (R)   9	  3.8%
Asn (N)  13	  5.4%
Asp (D)   6	  2.5%
Cys (C)   4	  1.7%
Gln (Q)  37	 15.5%
Glu (E)   6	  2.5%
Gly (G)   9	  3.8%
His (H)  12	  5.0%
Ile (I)  11	  4.6%
Leu (L)   9	  3.8%
Lys (K)   6	  2.5%
Met (M)  11	  4.6%
Phe (F)   5	  2.1%
Pro (P)  30	 12.6%
Ser (S)  22	  9.2%
Thr (T)  14	  5.9%
Trp (W)   4	  1.7%
Tyr (Y)  12	  5.0%
Val (V)   6	  2.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 12
Total number of positively charged residues (Arg + Lys): 15

Atomic composition:

Carbon      C	      1196
Hydrogen    H	      1808
Nitrogen    N	       350
Oxygen      O	       362
Sulfur      S	        15

Formula: C1196H1808N350O362S15
Total number of atoms: 3731

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    40130
Abs 0.1% (=1 g/l)   1.467, assuming all pairs of Cys residues form cystines


Ext. coefficient    39880
Abs 0.1% (=1 g/l)   1.457, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 62.41
This classifies the protein as unstable.



Aliphatic index: 45.36

Grand average of hydropathicity (GRAVY): -1.005