ProtParam
User-provided sequence:
10 20 30 40 50 60
MHSTGEPQRG PAGPYHHPMP YHVDQIPTTS HPSWHQAPHP GLPTPPYHPQ QHPNYISMQP
70 80 90 100 110 120
PPSQQQQMTP QALSTQQQQQ VQQQQQRQLY SSPSPSRGPA APAQNQKFRT EQWINNQAWP
130 140 150 160 170 180
YSQSPAPPVA PSVMSYHQGD DRMSMLSVNT TMTNQFPDSQ RCYSSNGSTC ENVNPEMMQM
190 200 210 220 230
NITQAAEQAI RFNFSYGLIH QIPCKDFIWQ KQYELGYDKI NLHKLIRQIC RTAYNKSLT
References and
documentation are available.
Number of amino acids: 239
Molecular weight: 27362.54
Theoretical pI: 8.66
Total number of negatively charged residues (Asp + Glu): 12
Total number of positively charged residues (Arg + Lys): 15
Atomic composition:
Carbon C 1196
Hydrogen H 1808
Nitrogen N 350
Oxygen O 362
Sulfur S 15
Formula: C
1196H
1808N
350O
362S
15
Total number of atoms: 3731
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 40130
Abs 0.1% (=1 g/l) 1.467, assuming all pairs of Cys residues form cystines
Ext. coefficient 39880
Abs 0.1% (=1 g/l) 1.457, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 62.41
This classifies the protein as unstable.
Aliphatic index: 45.36
Grand average of hydropathicity (GRAVY): -1.005