ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MHTAKPVKRY FNLKSSRIGM NPQTKTFEGA INRLNSLQSN AKVLEVLRKR GKIPNDQSMV 

        70         80         90        100        110        120 
EMRHWLRCIG YQPSDLNRLN VIHVAGTKGK GSTCAFTSSI LQQIQKSGER SIPKCIGMYT 

       130        140        150        160        170        180 
SPHLRSVCER IQLNGKPISQ ELFTKYFFDV WERLENAVGS DSEKPMYFRF LTLMAWHVFI 

       190        200        210        220        230        240 
SENVDAAIIE VGIGGEYDST NLIEKPYATA VTSLGLDHTS LLGNTIAEIA WQKAGIYKES 

       250        260        270        280        290        300 
AIALTCEQAP EAMNVLKNRA AERNTSLKVV IPPAELTPDM IGLSGVHQLG NTSLAVSLVQ 

       310        320        330        340        350        360 
EFYEKAGCPF DRDPYQDPAI LDGLKYVKWP GRCQIEEINN IKWCFDGAHT KESLEATGLW 

       370        380        390        400        410        420 
LASKKNLYED ADARILLFNQ QSRDDPIALL RSFLKGLESS GTGISFTHVI FSTNVTWKDA 

       430        440        450        460        470        480 
GYNPELLSIN TITDNKPVLH VQEDLCKWWK ESKGTTSEAT VAPTIQEAIE TVMSIKQKSR 

       490        500 
NTFVCVTGSL HLTGGVFVVL DQAVF 

References and documentation are available.

Number of amino acids: 505

Molecular weight: 56317.49

Theoretical pI: 7.56

Amino acid composition: 
Ala (A)  35	  6.9%
Arg (R)  21	  4.2%
Asn (N)  26	  5.1%
Asp (D)  21	  4.2%
Cys (C)  10	  2.0%
Gln (Q)  21	  4.2%
Glu (E)  33	  6.5%
Gly (G)  33	  6.5%
His (H)  11	  2.2%
Ile (I)  35	  6.9%
Leu (L)  47	  9.3%
Lys (K)  34	  6.7%
Met (M)  10	  2.0%
Phe (F)  19	  3.8%
Pro (P)  21	  4.2%
Ser (S)  39	  7.7%
Thr (T)  34	  6.7%
Trp (W)  10	  2.0%
Tyr (Y)  13	  2.6%
Val (V)  32	  6.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 54
Total number of positively charged residues (Arg + Lys): 55

Atomic composition:

Carbon      C	      2513
Hydrogen    H	      3971
Nitrogen    N	       681
Oxygen      O	       747
Sulfur      S	        20

Formula: C2513H3971N681O747S20
Total number of atoms: 7932

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    74995
Abs 0.1% (=1 g/l)   1.332, assuming all pairs of Cys residues form cystines


Ext. coefficient    74370
Abs 0.1% (=1 g/l)   1.321, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 39.39
This classifies the protein as stable.



Aliphatic index: 88.63

Grand average of hydropathicity (GRAVY): -0.223