ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MHVEQTRINM QSQGQSHQLP SAAHILLDQN PNSTGSNLVV KQNDIQSLSS VGLLELAHRE 

        70         80         90        100        110        120 
YQAVDYESAE KHCMQLWRQD STNTGVLLLL SSIHFQCRRL DKSAQFSTLA IKQNPVLAEA 

       130        140        150        160        170        180 
YSNLGNVFKE RGQLQEALDN YRRAVRLKPD FIDGYINLAA ALVAARDMES AVQAYITALQ 

       190        200        210        220        230        240 
YNPDLYCVRS DLGNLLKALG RLEEAKACYL KAIETCPGFA VAWSNLGCVF NAQGEIWLAI 

       250        260        270        280        290        300 
HHFEKAVTLD PNFLDAYINL GNVLKEARIF DRAVAAYLRA LNLSPNNAVV HGNLACVYYE 

       310        320        330        340        350        360 
QGLIDLAIDT YRRAIELQPN FPDAYCNLAN ALKEKGQVKE AEDCYNTALR LCSNHADSLN 

       370        380        390        400        410        420 
NLANIKREQG YIEEATRLYL KALEVFPDFA AAHSNLASVL QQQGKLKEAL MHYKEAIRIQ 

       430        440        450        460        470        480 
PTFADAYSNM GNTLKELQDV SGALQCYTRA IQINPAFADA HSNLASIHKD SGNIPEAIQS 

       490        500        510        520        530        540 
YRTALKLKPD FPDAYCNLAH CLQIVCDWTD YDIRMKKLVS IVTEQLEKNR LPSVHPHHSM 

       550        560        570        580        590        600 
LYPLTHDCRK AIAARHANLC LEKVHVLHKK PYNFLKKLPT KGRLRIGYLS SDFGNHPTSH 

       610        620        630        640        650        660 
LMQSVPGLHD RSKVEIFCYA LSPDDGTTFR HKISRESENF VDLSQIPCNG KAADKIFNDG 

       670        680        690        700        710        720 
IHILVNMNGY TKGARNEIFA LRPAPIQVMW LGYPGTSGAS FMDYIITDSV TSPLELAYQY 

       730        740        750        760        770        780 
SEKLSYMPHT YFIGDHKQMF PHLKERIIVC DKQQSSVVDN VTVINATDLS PLVENTDVKE 

       790        800        810        820        830        840 
IKEVVNAQKP VEITHKVAEL PNTTQIVSMI ATGQVQTSLN GVVVQNGLAT TQTNNKAATG 

       850        860        870        880        890        900 
EEVPQNIVIT TRRQYMLPDD AVVYCNFNQL YKIDPQTLES WVEILKNVPK SVLWLLRFPA 

       910        920        930        940        950        960 
VGEQNIKKTV SDFGISPDRV IFSNVAAKEE HVRRGQLADI CLDTPLCNGH TTSMDVLWTG 

       970        980        990       1000       1010 
TPVVTLPGET LASRVAASQL ATLGCPELIA RTREEYQNIA IRLGTKKKKK K 

References and documentation are available.

Number of amino acids: 1011

Molecular weight: 113022.15

Theoretical pI: 7.03

Amino acid composition: 
Ala (A)  95	  9.4%
Arg (R)  45	  4.5%
Asn (N)  64	  6.3%
Asp (D)  52	  5.1%
Cys (C)  23	  2.3%
Gln (Q)  54	  5.3%
Glu (E)  54	  5.3%
Gly (G)  46	  4.5%
His (H)  33	  3.3%
Ile (I)  59	  5.8%
Leu (L) 109	 10.8%
Lys (K)  59	  5.8%
Met (M)  17	  1.7%
Phe (F)  29	  2.9%
Pro (P)  44	  4.4%
Ser (S)  60	  5.9%
Thr (T)  53	  5.2%
Trp (W)   8	  0.8%
Tyr (Y)  39	  3.9%
Val (V)  68	  6.7%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 106
Total number of positively charged residues (Arg + Lys): 104

Atomic composition:

Carbon      C	      5017
Hydrogen    H	      7947
Nitrogen    N	      1397
Oxygen      O	      1494
Sulfur      S	        40

Formula: C5017H7947N1397O1494S40
Total number of atoms: 15895

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   103485
Abs 0.1% (=1 g/l)   0.916, assuming all pairs of Cys residues form cystines


Ext. coefficient   102110
Abs 0.1% (=1 g/l)   0.903, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 37.04
This classifies the protein as stable.



Aliphatic index: 93.71

Grand average of hydropathicity (GRAVY): -0.244