ProtParam
User-provided sequence:
10 20 30 40 50 60
MHWNIISKEQ SSSSVSLPTL DSSEPCHIEV ILRAIPEKGL QNNESTFKID PYENTVLFRT
70 80 90 100 110 120
NNPLHETTKE THSTFQFDKV FDANATQEDV QKFLVHPIIN DVLNGYNGTV ITYGPSFSGK
130 140 150 160 170 180
SYSLIGSKES EGILPNICKT LFDTLEKNEE TKGDSFSVSV LAFEIYMEKT YDLLVPLPER
190 200 210 220 230 240
KPLKLHRSSS KMDLEIKDIC PAHVGSYEDL RSYIQAVQNV GNRMACGDKT ERSRSHLVFQ
250 260 270 280 290 300
LHVEQRNRKD DILKNSSLYL VDLHGAEKFD KRTESTLSQD ALKKLNQSIE ALKNTVRSLS
310 320 330 340 350 360
MKERDSAYSA KGSHSSAYRE SQLTEVLKDS LGGNRKTKVI LTCFLSNVPT TLSTLEFGDS
370 380 390 400 410 420
IRQINNKVTD NTTGLNLKKK MDLFIQDMKI KDDNYVAQIN ILKAEIDSLK SLHNKSLPED
430 440 450 460 470 480
DEKKMLENTK KENIKLKLQL DSITQLLSSS TNEDPNNRID EEVSEILTKR CEQIAQLELS
490 500 510 520 530 540
FDRQMNSNSK LQQELEYKKS KEEALESMNV RLLEQIQLQE REIQELLTTN AILKGELETH
550 560 570 580 590 600
TKLTETRSER IKSLESSVKE LSLNKSAIPS PRRGSMSSSS GNTMLHIEEG SEISNSPWSA
610 620 630 640 650
NTSSKPLVWG ARKVSSSSIA TTGSQESFVA RPFKKGLNLH SIKVTSSTPK SPSSGS
References and
documentation are available.
Number of amino acids: 656
Molecular weight: 73799.23
Theoretical pI: 6.48
Total number of negatively charged residues (Asp + Glu): 91
Total number of positively charged residues (Arg + Lys): 87
Atomic composition:
Carbon C 3211
Hydrogen H 5220
Nitrogen N 898
Oxygen O 1051
Sulfur S 18
Formula: C
3211H
5220N
898O
1051S
18
Total number of atoms: 10398
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 36245
Abs 0.1% (=1 g/l) 0.491, assuming all pairs of Cys residues form cystines
Ext. coefficient 35870
Abs 0.1% (=1 g/l) 0.486, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 54.16
This classifies the protein as unstable.
Aliphatic index: 83.35
Grand average of hydropathicity (GRAVY): -0.634