ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MIAAGAKSLL GLSMASPKGI FDSNSMSNSR SVVVVRACVS MDGSQTLSHN KNGSIPEVKS 

        70         80         90        100        110        120 
INGHTGQKQG PLSTVGNSTN IKWHECSVEK VDRQRLLDQK GCVIWVTGLS GSGKSTLACA 

       130        140        150        160        170        180 
LNQMLYQKGK LCYILDGDNV RHGLNRDLSF KAEDRAENIR RVGEVAKLFA DAGIICIASL 

       190        200        210        220        230        240 
ISPYRTDRDA CRSLLPEGDF VEVFMDVPLS VCEARDPKGL YKLARAGKIK GFTGIDDPYE 

       250        260        270 
PPLNCEISLG REGGTSPIEM AEKVVGYLDN KGYLQA 

References and documentation are available.

Number of amino acids: 276

Molecular weight: 29787.09

Theoretical pI: 8.31

Amino acid composition: 
Ala (A)  19	  6.9%
Arg (R)  15	  5.4%
Asn (N)  13	  4.7%
Asp (D)  17	  6.2%
Cys (C)   9	  3.3%
Gln (Q)   8	  2.9%
Glu (E)  14	  5.1%
Gly (G)  28	 10.1%
His (H)   4	  1.4%
Ile (I)  16	  5.8%
Leu (L)  26	  9.4%
Lys (K)  19	  6.9%
Met (M)   7	  2.5%
Phe (F)   6	  2.2%
Pro (P)  11	  4.0%
Ser (S)  26	  9.4%
Thr (T)   9	  3.3%
Trp (W)   2	  0.7%
Tyr (Y)   7	  2.5%
Val (V)  20	  7.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 31
Total number of positively charged residues (Arg + Lys): 34

Atomic composition:

Carbon      C	      1293
Hydrogen    H	      2099
Nitrogen    N	       371
Oxygen      O	       402
Sulfur      S	        16

Formula: C1293H2099N371O402S16
Total number of atoms: 4181

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    21930
Abs 0.1% (=1 g/l)   0.736, assuming all pairs of Cys residues form cystines


Ext. coefficient    21430
Abs 0.1% (=1 g/l)   0.719, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 32.41
This classifies the protein as stable.



Aliphatic index: 87.25

Grand average of hydropathicity (GRAVY): -0.223