ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MIADIKKDAQ ERMGKCVDAT KNQMAKVRTG RAHPSLLDSI QVSYYGTMTP LNQVANVGVE 

        70         80         90        100        110        120 
DARTLSVTVF DRSAIQAVEK AIMSSDLGLN PMSAGATLRI PLPALTEERR KDFIKVVRAE 

       130        140        150        160        170        180 
AEGGRVAIRN VRRDAISDVK KLEKAKECTE DDVRRFEDEV QKFTDAHIKK VDEILAAKEI 


ELMEV 

References and documentation are available.

Number of amino acids: 185

Molecular weight: 20586.68

Theoretical pI: 6.76

Amino acid composition: 
Ala (A)  21	 11.4%
Arg (R)  15	  8.1%
Asn (N)   5	  2.7%
Asp (D)  15	  8.1%
Cys (C)   2	  1.1%
Gln (Q)   6	  3.2%
Glu (E)  16	  8.6%
Gly (G)   8	  4.3%
His (H)   2	  1.1%
Ile (I)  12	  6.5%
Leu (L)  12	  6.5%
Lys (K)  16	  8.6%
Met (M)   7	  3.8%
Phe (F)   4	  2.2%
Pro (P)   5	  2.7%
Ser (S)   9	  4.9%
Thr (T)  10	  5.4%
Trp (W)   0	  0.0%
Tyr (Y)   2	  1.1%
Val (V)  18	  9.7%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 31
Total number of positively charged residues (Arg + Lys): 31

Atomic composition:

Carbon      C	       888
Hydrogen    H	      1485
Nitrogen    N	       261
Oxygen      O	       280
Sulfur      S	         9

Formula: C888H1485N261O280S9
Total number of atoms: 2923

Extinction coefficients:

This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient     3105
Abs 0.1% (=1 g/l)   0.151, assuming all pairs of Cys residues form cystines


Ext. coefficient     2980
Abs 0.1% (=1 g/l)   0.145, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 42.50
This classifies the protein as unstable.



Aliphatic index: 90.16

Grand average of hydropathicity (GRAVY): -0.372