ProtParam
User-provided sequence:
10 20 30 40 50 60
MIAGATAPSS QHEILIASNL IKKPSTSQNK TPTAQSSSGN NGAADGAPQG YHHHHHHHRH
70 80 90 100 110 120
LWWPRTTDHQ YWCVLRKNQF AYYKTRDERE AISVIPRFDI LNFKISELDG ILTVYTPSKD
130 140 150 160 170 180
LIFKFPRGQN EKVGMELMHN WKIALEKFLS SPSGNESVTT GSDYDEEEDD DDLIVVDEKA
190 200 210 220 230 240
GPSSSKHSCS LTMDEQLSRE DKEFYRMFDP RNAEHQVCSG ILYTKVKKKK LFNRAKWQKF
250 260 270 280 290 300
NVELTNTSFN LYSFKTGKLK KSIKLDKIID CIELDNNSKM KNDDTNFALI TFDERLSFKA
310 320
ANDQDMVDWI INFKSGILIR KKLKAENI
References and
documentation are available.
Number of amino acids: 328
Molecular weight: 37739.64
Theoretical pI: 8.17
Total number of negatively charged residues (Asp + Glu): 45
Total number of positively charged residues (Arg + Lys): 47
Atomic composition:
Carbon C 1676
Hydrogen H 2611
Nitrogen N 465
Oxygen O 507
Sulfur S 11
Formula: C
1676H
2611N
465O
507S
11
Total number of atoms: 5270
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 46660
Abs 0.1% (=1 g/l) 1.236, assuming all pairs of Cys residues form cystines
Ext. coefficient 46410
Abs 0.1% (=1 g/l) 1.230, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 26.80
This classifies the protein as stable.
Aliphatic index: 72.29
Grand average of hydropathicity (GRAVY): -0.711