ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MIAPDSQRLF GSFDEQFKDL KLDSVDTENN NTHGVSTILD SSPASVNNNT NGAVAASVNT 

        70         80         90        100        110        120 
VPGSTFRSNT PLLGGRHPLS RTSSLIDSIG IQRAASPFSS MKEPFIPQSS GVMSSSFWHG 

       130        140        150        160        170        180 
DHPESRVSTP VQQHPLLQRN ESSSSFSYAA NLGVNLSTHS LAVDITPLST PTAAQSHVNL 

       190        200        210        220        230        240 
FPSSDIPPNM SMNGMSQLPA PVSVESSWRY IDTQGQIHGP FTTQMMSQWY IGGYFASTLQ 

       250        260        270        280        290        300 
ISRLGSTPET LGINDIFITL GELMTKLEKY DTDPFTTFDK LHVQTTSSDS INLNLAPYAS 

       310        320        330        340        350        360 
GVAATGTIKA TENDIFKPLT HDNIWDMDGG TTSKGVDIKL ASATTISQTD ESHKQEYKST 

       370        380        390        400        410        420 
TMLEKGKKEK SESVAKALLD EQEKRNRELK RKEEARLSKK QKQKEDDLLK KQKEQKEQKE 

       430        440        450        460        470        480 
KEALEAEKQK KSEKTKKDTQ TQTEGFKTSK DLPSLNSSSA NPAPWASKVK VNNAIETSIK 

       490        500        510        520        530        540 
NGVSSTGKKK GEPLGLQQRN SKEEKQKEEL KSVLNWANKS SLPSNQTIDI KSQFQKSPKG 

       550        560        570        580        590        600 
MKESSPLKEL EDPNFIEEQK KLWEKVQSSS KQVKSTSSAS TTTSSWTTVT SKGKAPIGTV 

       610        620        630        640        650        660 
VSPYSKTNTS LNSSLTAKTS TTSTTTTFAS MNNVSPRQEF IKWCKSQMKL NSGITNNNVL 

       670        680        690        700        710        720 
ELLLSLPTGP ESKELIQETI YANSDVMDGR RFATEFIKRR VACEKQGDDP LSWNEALALS 

       730        740 
GNDDDGWEFQ VVSKKKGRKH 

References and documentation are available.

Number of amino acids: 740

Molecular weight: 81396.95

Theoretical pI: 8.83

Amino acid composition: 
Ala (A)  40	  5.4%
Arg (R)  20	  2.7%
Asn (N)  42	  5.7%
Asp (D)  36	  4.9%
Cys (C)   2	  0.3%
Gln (Q)  40	  5.4%
Glu (E)  50	  6.8%
Gly (G)  39	  5.3%
His (H)  12	  1.6%
Ile (I)  32	  4.3%
Leu (L)  57	  7.7%
Lys (K)  73	  9.9%
Met (M)  15	  2.0%
Phe (F)  23	  3.1%
Pro (P)  37	  5.0%
Ser (S) 102	 13.8%
Thr (T)  67	  9.1%
Trp (W)  11	  1.5%
Tyr (Y)   9	  1.2%
Val (V)  33	  4.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 86
Total number of positively charged residues (Arg + Lys): 93

Atomic composition:

Carbon      C	      3538
Hydrogen    H	      5678
Nitrogen    N	       990
Oxygen      O	      1173
Sulfur      S	        17

Formula: C3538H5678N990O1173S17
Total number of atoms: 11396

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    74035
Abs 0.1% (=1 g/l)   0.910, assuming all pairs of Cys residues form cystines


Ext. coefficient    73910
Abs 0.1% (=1 g/l)   0.908, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 46.65
This classifies the protein as unstable.



Aliphatic index: 65.24

Grand average of hydropathicity (GRAVY): -0.753