ProtParam
User-provided sequence:
10 20 30 40 50 60
MIATPIRLAK SAYEPMIKFV GTRHPLVKHA TEVVVHPCAT NGMLPGSKEC IPVSKFMENY
70 80 90 100 110 120
KPFRVVPIKH SANAGLSSSK TSVFVNRPLQ KDELASIFEL PARFRYKPIN EHELESINSG
GAW
References and
documentation are available.
Number of amino acids: 123
Molecular weight: 13688.96
Theoretical pI: 9.55
Total number of negatively charged residues (Asp + Glu): 10
Total number of positively charged residues (Arg + Lys): 16
Atomic composition:
Carbon C 618
Hydrogen H 979
Nitrogen N 169
Oxygen O 170
Sulfur S 6
Formula: C
618H
979N
169O
170S
6
Total number of atoms: 1942
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 10095
Abs 0.1% (=1 g/l) 0.737, assuming all pairs of Cys residues form cystines
Ext. coefficient 9970
Abs 0.1% (=1 g/l) 0.728, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 26.38
This classifies the protein as stable.
Aliphatic index: 82.44
Grand average of hydropathicity (GRAVY): -0.185