ProtParam
User-provided sequence:
10 20 30 40 50 60
MIATSRAVNM NKESKHKKAV AKPCRERQTS VTRAMRPAVA RDPRRLSTSS SPSSSPMSAQ
70 80 90 100 110 120
RRLSREEIIN EMEKEQDAIV VRLLREIETL KEENSRLKNQ LHHPVPARRS SPFFEGESAI
130 140 150 160 170 180
LDDDDCNYGY TLDTPKLKLT DGASRHTVLP LTPKDSMTHI SHSARRSSRN ASISNGTSIS
190 200 210 220 230 240
DTIFPIETKI HSAPTTNRNL PSADLPHHTL LPRSLSGISS SDLTESGALL HDRRRRSSNY
250 260
SLDGSNSLKA DLMAKRFQTG SLK
References and
documentation are available.
Number of amino acids: 263
Molecular weight: 29262.94
Theoretical pI: 9.99
Total number of negatively charged residues (Asp + Glu): 30
Total number of positively charged residues (Arg + Lys): 42
Atomic composition:
Carbon C 1241
Hydrogen H 2058
Nitrogen N 396
Oxygen O 403
Sulfur S 9
Formula: C
1241H
2058N
396O
403S
9
Total number of atoms: 4107
Extinction coefficients:
This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 4595
Abs 0.1% (=1 g/l) 0.157, assuming all pairs of Cys residues form cystines
Ext. coefficient 4470
Abs 0.1% (=1 g/l) 0.153, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 68.89
This classifies the protein as unstable.
Aliphatic index: 72.40
Grand average of hydropathicity (GRAVY): -0.775