ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MIDISHGHTS CSSHRSSSLP SVFWKIHLSP PLPSFKFKKS TESTQRQTIP KFSSPASSLA 

        70         80         90        100 
PSTPLWSLSS CLLVSEVSFW PGAGFLYNDL VCLDREDHCL SNSASHSCG 

References and documentation are available.

Number of amino acids: 109

Molecular weight: 11897.44

Theoretical pI: 7.71

Amino acid composition: 
Ala (A)   4	  3.7%
Arg (R)   3	  2.8%
Asn (N)   2	  1.8%
Asp (D)   4	  3.7%
Cys (C)   5	  4.6%
Gln (Q)   2	  1.8%
Glu (E)   3	  2.8%
Gly (G)   4	  3.7%
His (H)   6	  5.5%
Ile (I)   4	  3.7%
Leu (L)  12	 11.0%
Lys (K)   5	  4.6%
Met (M)   1	  0.9%
Phe (F)   6	  5.5%
Pro (P)   9	  8.3%
Ser (S)  26	 23.9%
Thr (T)   5	  4.6%
Trp (W)   3	  2.8%
Tyr (Y)   1	  0.9%
Val (V)   4	  3.7%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 7
Total number of positively charged residues (Arg + Lys): 8

Atomic composition:

Carbon      C	       528
Hydrogen    H	       808
Nitrogen    N	       142
Oxygen      O	       160
Sulfur      S	         6

Formula: C528H808N142O160S6
Total number of atoms: 1644

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    18240
Abs 0.1% (=1 g/l)   1.533, assuming all pairs of Cys residues form cystines


Ext. coefficient    17990
Abs 0.1% (=1 g/l)   1.512, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 65.55
This classifies the protein as unstable.



Aliphatic index: 71.56

Grand average of hydropathicity (GRAVY): -0.149