ProtParam
User-provided sequence:
10 20 30 40 50 60
MIEKCIGAHR FRRLQRFMRQ GKVTILCLVL TVIVLRGTIG AGKFGTPEKD IEEIREHFFY
70 80 90 100 110 120
TRKRGEPHRV LVEVSSKTTS SEDGGNGGNS YETFDINKLF VDEGDEEKSR DRTNKPYSLG
130 140 150 160 170 180
PKISDWDEQR RDWLKQNPSF PNFVAPNKPR VLLVTGSAPK PCENPVGDHY LLKSIKNKID
190 200 210 220 230 240
YCRIHGIEIF YNMALLDAEM AGFWAKLPLI RKLLLSHPEI EFLWWMDSDA MFTDMVFELP
250 260 270 280 290 300
WERYKDYNLV MHGWNEMVYD QKNWIGLNTG SFLLRNSQWS LDLLDAWAPM GPKGKIREEA
310 320 330 340 350 360
GKVLTRELKD RPAFEADDQS AMVYLLATER EKWGGKVYLE SGYYLHGYWG ILVDRYEEMI
370 380 390 400 410 420
ENHKPGFGDH RWPLVTHFVG CKPCGKFGDY PVERCLRQMD RAFNFGDNQI LQMYGFTHKS
430 440 450 460
LGSRRVKPTR NQTDRPLDAK DEFGLLHPPF KAAKLSTTTT
References and
documentation are available.
Number of amino acids: 460
Molecular weight: 53382.10
Theoretical pI: 8.29
Total number of negatively charged residues (Asp + Glu): 63
Total number of positively charged residues (Arg + Lys): 66
Atomic composition:
Carbon C 2408
Hydrogen H 3695
Nitrogen N 659
Oxygen O 677
Sulfur S 21
Formula: C
2408H
3695N
659O
677S
21
Total number of atoms: 7460
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 97205
Abs 0.1% (=1 g/l) 1.821, assuming all pairs of Cys residues form cystines
Ext. coefficient 96830
Abs 0.1% (=1 g/l) 1.814, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 37.78
This classifies the protein as stable.
Aliphatic index: 73.96
Grand average of hydropathicity (GRAVY): -0.563