ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MIEKCIGAHR FRRLQRFMRQ GKVTILCLVL TVIVLRGTIG AGKFGTPEKD IEEIREHFFY 

        70         80         90        100        110        120 
TRKRGEPHRV LVEVSSKTTS SEDGGNGGNS YETFDINKLF VDEGDEEKSR DRTNKPYSLG 

       130        140        150        160        170        180 
PKISDWDEQR RDWLKQNPSF PNFVAPNKPR VLLVTGSAPK PCENPVGDHY LLKSIKNKID 

       190        200        210        220        230        240 
YCRIHGIEIF YNMALLDAEM AGFWAKLPLI RKLLLSHPEI EFLWWMDSDA MFTDMVFELP 

       250        260        270        280        290        300 
WERYKDYNLV MHGWNEMVYD QKNWIGLNTG SFLLRNSQWS LDLLDAWAPM GPKGKIREEA 

       310        320        330        340        350        360 
GKVLTRELKD RPAFEADDQS AMVYLLATER EKWGGKVYLE SGYYLHGYWG ILVDRYEEMI 

       370        380        390        400        410        420 
ENHKPGFGDH RWPLVTHFVG CKPCGKFGDY PVERCLRQMD RAFNFGDNQI LQMYGFTHKS 

       430        440        450        460 
LGSRRVKPTR NQTDRPLDAK DEFGLLHPPF KAAKLSTTTT 

References and documentation are available.

Number of amino acids: 460

Molecular weight: 53382.10

Theoretical pI: 8.29

Amino acid composition: 
Ala (A)  20	  4.3%
Arg (R)  32	  7.0%
Asn (N)  19	  4.1%
Asp (D)  30	  6.5%
Cys (C)   7	  1.5%
Gln (Q)  11	  2.4%
Glu (E)  33	  7.2%
Gly (G)  37	  8.0%
His (H)  13	  2.8%
Ile (I)  21	  4.6%
Leu (L)  44	  9.6%
Lys (K)  34	  7.4%
Met (M)  14	  3.0%
Phe (F)  24	  5.2%
Pro (P)  25	  5.4%
Ser (S)  21	  4.6%
Thr (T)  22	  4.8%
Trp (W)  13	  2.8%
Tyr (Y)  17	  3.7%
Val (V)  23	  5.0%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 63
Total number of positively charged residues (Arg + Lys): 66

Atomic composition:

Carbon      C	      2408
Hydrogen    H	      3695
Nitrogen    N	       659
Oxygen      O	       677
Sulfur      S	        21

Formula: C2408H3695N659O677S21
Total number of atoms: 7460

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    97205
Abs 0.1% (=1 g/l)   1.821, assuming all pairs of Cys residues form cystines


Ext. coefficient    96830
Abs 0.1% (=1 g/l)   1.814, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 37.78
This classifies the protein as stable.



Aliphatic index: 73.96

Grand average of hydropathicity (GRAVY): -0.563