ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MIFKIEDVTV YFPYDNIYPE QYEYMVELKR ALDAKGHCLL EMPTGTGKTI ALLSLITSYR 

        70         80         90        100        110        120 
LSRPDSPIKL VYCTRTVHEM EKTLGELKLL HDYQVRHLGT QAKILALGLS SRKNLCVNTK 

       130        140        150        160        170        180 
VLAAENRDSV DAACRKRTAS WVRALSTENP NVELCDFFEN YEKAAENALL PPGVYTLEDL 

       190        200        210        220        230        240 
RAFGKNRGWC PYFLARHMIQ FANVIVYSYQ YLLDPKVAGF ISKELQKESV VVFDEAHNID 

       250        260        270        280        290        300 
NVCIEALSVS VRRVTLEGAN RNLNKIRQEI DRFKATDAGR LRAEYNRLVE GLALRGDLSG 

       310        320        330        340        350        360 
GDQWLANPAL PHDILKEAVP GNIRRAEHFV HVLRRLLQYL GVRLDTENVE KESPVSFVSS 

       370        380        390        400        410        420 
LNSQAGIEQK TLKFCYDRLQ SLMLTLEITD TDEFLPIQTV CDFATLVGTY ARGFSIIIEP 

       430        440        450        460        470        480 
YDERMPHIPD PILQLSCHDA SLAIKPVFDR FQSVVITSGT LSPIDLYPRL LNFTPVVSRS 

       490        500        510        520        530        540 
FKMSMTRDCI CPMVLTRGSD QLPVSTKFDM RSDPGVVRNY GKLLVEMVSI VPDGVVCFFV 

       550        560        570        580        590        600 
SYSYMDGIIA TWNETGILKE IMQQKLVFIE TQDVVETTLA LDNYRRACDC GRGAVFFSVA 

       610        620        630        640        650        660 
RGKVAEGIDF DRHYGRLVVM YGVPFQYTLS KILRARLEYL HDTFQIKEGD FLTFDALRQA 

       670        680        690        700        710        720 
AQCVGRVIRS KADYGMMIFA DKRYSRHDKR SKLPGWILSH LRDAHLNLST DMAIHIAREF 

       730        740        750 
LRKMAQPYDK AGTMGRKTLL TQEDLEKMAE TGVQDMAY 

References and documentation are available.

Number of amino acids: 758

Molecular weight: 86235.52

Theoretical pI: 7.23

Amino acid composition: 
Ala (A)  53	  7.0%
Arg (R)  55	  7.3%
Asn (N)  25	  3.3%
Asp (D)  49	  6.5%
Cys (C)  16	  2.1%
Gln (Q)  26	  3.4%
Glu (E)  45	  5.9%
Gly (G)  41	  5.4%
His (H)  17	  2.2%
Ile (I)  42	  5.5%
Leu (L)  84	 11.1%
Lys (K)  39	  5.1%
Met (M)  22	  2.9%
Phe (F)  33	  4.4%
Pro (P)  30	  4.0%
Ser (S)  43	  5.7%
Thr (T)  42	  5.5%
Trp (W)   5	  0.7%
Tyr (Y)  32	  4.2%
Val (V)  59	  7.8%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 94
Total number of positively charged residues (Arg + Lys): 94

Atomic composition:

Carbon      C	      3854
Hydrogen    H	      6104
Nitrogen    N	      1052
Oxygen      O	      1115
Sulfur      S	        38

Formula: C3854H6104N1052O1115S38
Total number of atoms: 12163

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    76180
Abs 0.1% (=1 g/l)   0.883, assuming all pairs of Cys residues form cystines


Ext. coefficient    75180
Abs 0.1% (=1 g/l)   0.872, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 45.02
This classifies the protein as unstable.



Aliphatic index: 94.39

Grand average of hydropathicity (GRAVY): -0.145