ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MIFVDRIDAL GRILTRILRH MATELRLNMR GDGFVKVEDL LNLNLKTCAN IQLNSHTIDE 

        70         80         90        100        110        120 
IREAVTRDNK KRFSLIDEDG ELLIRANQGH SITTVESEKL LKPILSPEEA PVCVHGTYRK 

       130        140        150        160        170        180 
NLESILASGL KRMNRMHVHF SCGLPTDGEV ISGVRRNVNV IIFLHIKKAL EDGIAFYISD 

       190        200        210 
NKVILTQGIV GVLPVDYFQK IESWPDRQSI PF 

References and documentation are available.

Number of amino acids: 212

Molecular weight: 24055.94

Theoretical pI: 7.78

Amino acid composition: 
Ala (A)   9	  4.2%
Arg (R)  16	  7.5%
Asn (N)  12	  5.7%
Asp (D)  13	  6.1%
Cys (C)   3	  1.4%
Gln (Q)   5	  2.4%
Glu (E)  14	  6.6%
Gly (G)  13	  6.1%
His (H)   7	  3.3%
Ile (I)  22	 10.4%
Leu (L)  24	 11.3%
Lys (K)  12	  5.7%
Met (M)   5	  2.4%
Phe (F)   8	  3.8%
Pro (P)   7	  3.3%
Ser (S)  12	  5.7%
Thr (T)  10	  4.7%
Trp (W)   1	  0.5%
Tyr (Y)   3	  1.4%
Val (V)  16	  7.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 27
Total number of positively charged residues (Arg + Lys): 28

Atomic composition:

Carbon      C	      1069
Hydrogen    H	      1744
Nitrogen    N	       304
Oxygen      O	       309
Sulfur      S	         8

Formula: C1069H1744N304O309S8
Total number of atoms: 3434

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    10095
Abs 0.1% (=1 g/l)   0.420, assuming all pairs of Cys residues form cystines


Ext. coefficient     9970
Abs 0.1% (=1 g/l)   0.414, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 46.05
This classifies the protein as unstable.



Aliphatic index: 110.75

Grand average of hydropathicity (GRAVY): -0.094