ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MIGAFKRNRG SSQSFAKECQ PSTLKANLEV AKELPFSDRR DFEDATQGYI GSLSDEQIIG 

        70         80         90        100        110        120 
PDGGVVWCMK SYGFLEPETP ANTVNPSLWR QAQLNAIHGL FKITDNVYQV RGLDISNMTI 

       130        140        150        160        170        180 
IEGNTSLIII DTLFTTETAQ ESLKLYYRHR PQKPVRTVIY THSHSDHYGG VKGIVKEADV 

       190        200        210        220        230        240 
KSGEVQIIAP VGFMESVVAE NILAGNAMHR RSQYQFGMLL SPSVKGHVDC GIGKAASHGT 

       250        260        270        280        290        300 
VTLIAPTIII EEPVEERTID GVDFVFQLAP GSEAPSEMLI YMPQQRVLNM AEDVTHHMHN 

       310        320        330        340        350        360 
LYALRGVEVR DGNQWAKYID AARVAFGSKT DVLIAQHHWP TTGQMRINEL LKKQRDMYKF 

       370        380        390        400        410        420 
IHDQTLRLLN QGYTSRDIAE TLRMPSSLEQ EWSTRGYYGT LSHNVKAVYQ KYLGWYDANP 

       430        440        450        460        470        480 
ANLNPLPPVA YAKKAVEYMG GADAVLARAY KDFQKGEFRW VASVVNQLVF ADPNNHQARE 

       490        500        510        520        530        540 
LCADALEQLG YQAEASTWRN AYLVGAMELR QGVPKRRSTG KRNNIAVLNN EMFFDFLAVR 

       550        560        570        580        590        600 
LNATKAEGKI IVSNWCFINS NERFVITLEN CALTYIQGWQ TDADATITLK RTTFEALLAN 

       610        620        630        640 
EITMVDFLRS KEVEIEGNRL RIEELLKLFD DFDQSFPVVE PMGGST 

References and documentation are available.

Number of amino acids: 646

Molecular weight: 72645.56

Theoretical pI: 6.02

Amino acid composition: 
Ala (A)  55	  8.5%
Arg (R)  36	  5.6%
Asn (N)  34	  5.3%
Asp (D)  32	  5.0%
Cys (C)   6	  0.9%
Gln (Q)  32	  5.0%
Glu (E)  44	  6.8%
Gly (G)  44	  6.8%
His (H)  16	  2.5%
Ile (I)  39	  6.0%
Leu (L)  54	  8.4%
Lys (K)  31	  4.8%
Met (M)  18	  2.8%
Phe (F)  26	  4.0%
Pro (P)  25	  3.9%
Ser (S)  36	  5.6%
Thr (T)  38	  5.9%
Trp (W)  10	  1.5%
Tyr (Y)  24	  3.7%
Val (V)  46	  7.1%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 76
Total number of positively charged residues (Arg + Lys): 67

Atomic composition:

Carbon      C	      3236
Hydrogen    H	      5053
Nitrogen    N	       893
Oxygen      O	       963
Sulfur      S	        24

Formula: C3236H5053N893O963S24
Total number of atoms: 10169

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    91135
Abs 0.1% (=1 g/l)   1.255, assuming all pairs of Cys residues form cystines


Ext. coefficient    90760
Abs 0.1% (=1 g/l)   1.249, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 42.57
This classifies the protein as unstable.



Aliphatic index: 85.31

Grand average of hydropathicity (GRAVY): -0.293