ProtParam
User-provided sequence:
10 20 30 40 50 60
MIGAVNSVEA VITSIQGLSG SPEDLSALHD LLRGAQDSLR AEPGVNFSTL DQLDASKHSL
70 80 90 100 110 120
GYLYFLEVLT CGPVSKEKAA YEIPIIARFI NSCDAGQIRL ASYKFVSLCK ILKDHVIALG
130 140 150 160 170 180
DPLRGVGPLL NAVQKLQVSS KRLTALHPDV LQLCLQAKSY KSGFSILSDD IVEIDQPRDF
190 200 210 220 230 240
FLYSYYGGMI CIGLKRFQKA LELLYNVVTA PMHQVNAIAL EAYKKYILVS LIHNGQFTNT
250 260 270 280 290 300
LPKCASTAAQ RSFKNYTGPY IELGNCYNDG KIGELEALVV ARNAEFEEDK NLGLVKQAVS
310 320 330 340 350 360
SLYKRNILRL TQKYLTLSLQ DIANMVQLGN AKEAEMHVLQ MIQDGQIHAL INQKDGMVRF
370 380 390 400 410 420
LEDPEQYKSS EMIEIMDSVI QRTIGLSKNL LAMDESLSCD PLYLGKVGRE RQRYDFGDDF
DTVPQKFSM
References and
documentation are available.
Number of amino acids: 429
Molecular weight: 47741.03
Theoretical pI: 5.96
Total number of negatively charged residues (Asp + Glu): 49
Total number of positively charged residues (Arg + Lys): 44
Atomic composition:
Carbon C 2131
Hydrogen H 3406
Nitrogen N 566
Oxygen O 636
Sulfur S 19
Formula: C
2131H
3406N
566O
636S
19
Total number of atoms: 6758
Extinction coefficients:
This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 28810
Abs 0.1% (=1 g/l) 0.603, assuming all pairs of Cys residues form cystines
Ext. coefficient 28310
Abs 0.1% (=1 g/l) 0.593, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 37.36
This classifies the protein as stable.
Aliphatic index: 102.98
Grand average of hydropathicity (GRAVY): -0.061