ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MIGEHKALVQ RITNETKIQI AISLNGGHIE LPESILDKNP TQENNIATQA TTSQVIDIHT 

        70         80         90        100        110        120 
GVGFLDHMIH ALAKHSGWSL IVECIGDLHI DDHHTTEDCG IALGEAFKQA LGQVRGVKRF 

       130        140        150        160        170        180 
GCGFAPLDEA LSRAVVDLSN RPYAVIDLGL KREKIGDLSC EMIPHFLESF IEAARLTVHI 

       190        200        210        220 
DCLRGFNDHH RSESAFKALA VAIREATAPN GTNDVPSTKG VLM 

References and documentation are available.

Number of amino acids: 223

Molecular weight: 24283.68

Theoretical pI: 5.76

Amino acid composition: 
Ala (A)  21	  9.4%
Arg (R)  10	  4.5%
Asn (N)   9	  4.0%
Asp (D)  14	  6.3%
Cys (C)   5	  2.2%
Gln (Q)   7	  3.1%
Glu (E)  15	  6.7%
Gly (G)  18	  8.1%
His (H)  13	  5.8%
Ile (I)  21	  9.4%
Leu (L)  21	  9.4%
Lys (K)  10	  4.5%
Met (M)   4	  1.8%
Phe (F)   8	  3.6%
Pro (P)   7	  3.1%
Ser (S)  12	  5.4%
Thr (T)  13	  5.8%
Trp (W)   1	  0.4%
Tyr (Y)   1	  0.4%
Val (V)  13	  5.8%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 29
Total number of positively charged residues (Arg + Lys): 20

Atomic composition:

Carbon      C	      1066
Hydrogen    H	      1708
Nitrogen    N	       306
Oxygen      O	       324
Sulfur      S	         9

Formula: C1066H1708N306O324S9
Total number of atoms: 3413

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient     7240
Abs 0.1% (=1 g/l)   0.298, assuming all pairs of Cys residues form cystines


Ext. coefficient     6990
Abs 0.1% (=1 g/l)   0.288, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 38.65
This classifies the protein as stable.



Aliphatic index: 99.78

Grand average of hydropathicity (GRAVY): -0.059