ProtParam
User-provided sequence:
10 20 30 40 50 60
MIGGFNSDMK EHIRRANKGE IHCHFPSHKS QNELTELLAN DTKMMILKKI KDAKYFSVIL
70 80 90 100 110 120
DSIPDVSRKE QMTFLIRCVD VSTCSPKIEE FFLTFLHIKD KREYTDNPGH RSDVESLTES
130 140 150 160 170 180
ETHGIGGFEF LFGMIIWYDL LAAVNIVSKS LQFEDMDLEV AISQLGGLVT YLKNYKETGF
190 200 210 220 230 240
EKAKVESTQI AIEMKIAPVF PKKSVKKKKQ FVEDVEKIDE SKIAEESFRI DYFINIMDQA
250 260 270 280 290 300
IMCIEIRFEQ FQVYEQIFGF LFGVKRLKVA EDDELRTSCM KLEASLKHDV HSDVDGEDLF
310 320 330 340 350 360
MELKLLKDVL PKEITKPVEV LKFLKIMDSC YPNTWIAYRI LLTIPVSVAL AERTFSKLKL
370 380
IKKYLRSTMS QERLNGLALI SV
References and
documentation are available.
Number of amino acids: 382
Molecular weight: 44120.45
Theoretical pI: 6.07
Total number of negatively charged residues (Asp + Glu): 59
Total number of positively charged residues (Arg + Lys): 54
Atomic composition:
Carbon C 1997
Hydrogen H 3179
Nitrogen N 505
Oxygen O 576
Sulfur S 20
Formula: C
1997H
3179N
505O
576S
20
Total number of atoms: 6277
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 26275
Abs 0.1% (=1 g/l) 0.596, assuming all pairs of Cys residues form cystines
Ext. coefficient 25900
Abs 0.1% (=1 g/l) 0.587, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 43.72
This classifies the protein as unstable.
Aliphatic index: 96.94
Grand average of hydropathicity (GRAVY): -0.147