ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MIGKVAGTAA IAGISFLAGK YSNDDLPIFR NVQSATNVPM NQIQVSEPMT VKPASLNADA 

        70         80         90        100        110        120 
MGPSRSAEIM KHGYPGFTNV RTYEDFVLSY DYKTRTAHWV CEHLTPERLK HAEGVDRKLC 

       130        140        150        160        170        180 
EFKPDITFPQ KFLSQNTDYK CSGFDRGHLA AAGNHRKSQL AVDQTFYLSN MSPQVGRGFN 

       190        200        210        220        230        240 
RDKWNDLEMH CRRVAKKMIN SYIITGPLYL PKLEGDGKKY IKYQVIGDNN VAVPTHFFKV 

       250        260        270        280        290        300 
ALFEVTPGKF ELESYILPNA VIEDTVEISK FHVPLDAVER SAGLEIFARL DPKSIVKENG 


AKKGGLLW 

References and documentation are available.

Number of amino acids: 308

Molecular weight: 34510.54

Theoretical pI: 8.84

Amino acid composition: 
Ala (A)  24	  7.8%
Arg (R)  14	  4.5%
Asn (N)  16	  5.2%
Asp (D)  17	  5.5%
Cys (C)   4	  1.3%
Gln (Q)   9	  2.9%
Glu (E)  17	  5.5%
Gly (G)  22	  7.1%
His (H)   9	  2.9%
Ile (I)  17	  5.5%
Leu (L)  23	  7.5%
Lys (K)  25	  8.1%
Met (M)   8	  2.6%
Phe (F)  16	  5.2%
Pro (P)  17	  5.5%
Ser (S)  18	  5.8%
Thr (T)  15	  4.9%
Trp (W)   3	  1.0%
Tyr (Y)  12	  3.9%
Val (V)  22	  7.1%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 34
Total number of positively charged residues (Arg + Lys): 39

Atomic composition:

Carbon      C	      1552
Hydrogen    H	      2417
Nitrogen    N	       421
Oxygen      O	       447
Sulfur      S	        12

Formula: C1552H2417N421O447S12
Total number of atoms: 4849

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    34630
Abs 0.1% (=1 g/l)   1.003, assuming all pairs of Cys residues form cystines


Ext. coefficient    34380
Abs 0.1% (=1 g/l)   0.996, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 27.83
This classifies the protein as stable.



Aliphatic index: 79.16

Grand average of hydropathicity (GRAVY): -0.343