ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MIIKALAIVS LCLASIQASK VGAPQLPKKH LVCYYDSASF VKEGLGKLVI DELEPALQFC 

        70         80         90        100        110        120 
DYLVYGYAGI ERDSHKAVSL NQQLDLDLGK GLYRTVTRLK RKYPNVKILL SVGGDKDIEL 

       130        140        150        160        170        180 
DKDAKELPNK YLELLESPTG RTRFVNTVYS LVKTYGFDGL DVAWQFPKNK PKKVHSGIGS 

       190        200        210        220        230        240 
LWKGFKKVFS GDSIVDEKSE EHKEQFTALL RDVKNAFRPD NLLLSTTVLP NVNSSLFYDI 

       250        260        270        280        290        300 
PAVVNYLDFV NLGTFDFFTP QRNPEVADYA APIYELSERN PEFNVAAQVK YWLRNNCPAS 

       310        320        330        340        350        360 
KINVGVATYG RPWKLTDDSG DTGVPPVKDV KDEAPVGGNT QVPGIYSWPE VCALLPNQNN 

       370        380        390        400        410        420 
AYLKGANAPL IKVQDPAKRF GSYAYRAADK KGDNGIWVSF EDPDTAADKA GYVRTENLGG 

       430        440        450 
VALFDLSYDD FRGLCTNEKY PILRAIKYRL TN 

References and documentation are available.

Number of amino acids: 452

Molecular weight: 50354.41

Theoretical pI: 7.99

Amino acid composition: 
Ala (A)  35	  7.7%
Arg (R)  18	  4.0%
Asn (N)  26	  5.8%
Asp (D)  34	  7.5%
Cys (C)   6	  1.3%
Gln (Q)  12	  2.7%
Glu (E)  21	  4.6%
Gly (G)  32	  7.1%
His (H)   4	  0.9%
Ile (I)  18	  4.0%
Leu (L)  48	 10.6%
Lys (K)  39	  8.6%
Met (M)   1	  0.2%
Phe (F)  19	  4.2%
Pro (P)  27	  6.0%
Ser (S)  25	  5.5%
Thr (T)  19	  4.2%
Trp (W)   6	  1.3%
Tyr (Y)  24	  5.3%
Val (V)  38	  8.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 55
Total number of positively charged residues (Arg + Lys): 57

Atomic composition:

Carbon      C	      2288
Hydrogen    H	      3555
Nitrogen    N	       597
Oxygen      O	       669
Sulfur      S	         7

Formula: C2288H3555N597O669S7
Total number of atoms: 7116

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    69135
Abs 0.1% (=1 g/l)   1.373, assuming all pairs of Cys residues form cystines


Ext. coefficient    68760
Abs 0.1% (=1 g/l)   1.366, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 29.49
This classifies the protein as stable.



Aliphatic index: 89.07

Grand average of hydropathicity (GRAVY): -0.312