ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MIIKSKLPNL DIPDKTFHDF LFHEWEKFRE DVALIDNDTF AQFTFQDVMQ KADFIAKSLV 

        70         80         90        100        110        120 
YLKVEKSEVV LLVMDWSPAA IYIALGASMA GAAIQVVSPK LQAWEMQFPV KESESRFVFS 

       130        140        150        160        170        180 
DPLGLHEIDK LMKTLNREYR IICTGSRDFA NGYPIIEDLA FAAKQNLPLP KIEPDTDIVY 

       190        200        210        220        230        240 
LPYSSGIHGK RKGIVTTHKV MVAKTMVMWN TSKHHEFRRG DTTLTMIPLH KQCGLDAILC 

       250        260        270        280        290        300 
ALLNGLTVVT EKNFCVHTFM TCIQRYKIRA VHLTPYLMNL MMFEAENHTY NIESLEWVVT 

       310        320        330        340        350        360 
GADAVTEELY EEFTEIFPSV KTVTQTYGMT EVGLICRTYN KNYSPSCGQL TANLEMKVLD 

       370        380        390        400        410        420 
ILTGKELGPR EKGQICVKGI AAESPYLNNP KATEEHFLDG WRRTGDIGFF DEEGNVHLVD 

       430        440        450        460        470        480 
KLKEMIKVFG YQVIPKEIET LLLTHQAVEE AAVVAINNEL SGERPVAFVV LKKGFVATED 

       490        500        510        520        530        540 
DLKDYVNKRV IRYKHLVRVN ITQFLPKSAC GTLLRRLLAE AAVMSVASIA EVEKEFVPEL 

       550 
PADMKSILTH 

References and documentation are available.

Number of amino acids: 550

Molecular weight: 62317.34

Theoretical pI: 5.87

Amino acid composition: 
Ala (A)  40	  7.3%
Arg (R)  20	  3.6%
Asn (N)  21	  3.8%
Asp (D)  27	  4.9%
Cys (C)   9	  1.6%
Gln (Q)  15	  2.7%
Glu (E)  45	  8.2%
Gly (G)  27	  4.9%
His (H)  16	  2.9%
Ile (I)  36	  6.5%
Leu (L)  54	  9.8%
Lys (K)  41	  7.5%
Met (M)  19	  3.5%
Phe (F)  27	  4.9%
Pro (P)  23	  4.2%
Ser (S)  23	  4.2%
Thr (T)  37	  6.7%
Trp (W)   6	  1.1%
Tyr (Y)  17	  3.1%
Val (V)  47	  8.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 72
Total number of positively charged residues (Arg + Lys): 61

Atomic composition:

Carbon      C	      2819
Hydrogen    H	      4443
Nitrogen    N	       725
Oxygen      O	       808
Sulfur      S	        28

Formula: C2819H4443N725O808S28
Total number of atoms: 8823

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    58830
Abs 0.1% (=1 g/l)   0.944, assuming all pairs of Cys residues form cystines


Ext. coefficient    58330
Abs 0.1% (=1 g/l)   0.936, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 33.12
This classifies the protein as stable.



Aliphatic index: 95.87

Grand average of hydropathicity (GRAVY): -0.050