ProtParam
User-provided sequence:
10 20 30 40 50 60
MIINRRILKS FEEISHSLEE SLREVAFDSQ QQLIQDVREE NEELSRLQDQ LQLIRSIVEK
70 80 90 100 110 120
ICISIKTDNI DSYCSVPFDL LYNICKDIAD PSSFEDGDLQ YLVSQAIFEY IILLCYYSVT
130 140 150 160 170 180
NECVQGLPAV YEAEQYYKTV SDSILKSFLY CLQNSVSTIR LLSQTVLKDV NKKKLSHQKW
190 200 210 220 230 240
SLKALSVDLL EKIRPRINKF MVIRNFRFVG LPKKPIEIAS LVSDIPRGIV HERLDMVTQS
250 260 270 280 290 300
SKYYTIKLGQ LITEFDQQPE ENGMFTEVHL PNYERRLKSL QDFFGLAMSD SNLLDVIRCS
310 320 330 340 350 360
AKFHKDHPLR RFTKPSILTR YWPSILLCLL YGPSSVMSLW NSRYFIQDFI KTNVVDFAKG
370 380 390 400 410 420
LILNWLWAPL KQVWSTVKHD EGSAISVTSQ ETLNSDMDSL TRMIVSFVVD NSDSTSNSPI
430 440 450 460 470 480
DPILLSTKVE HGDLTEFMEI YETQLHHPIK NIATGGLVRS LLIQLQKTKV DGSMALNGID
490 500 510 520 530 540
KMLKSQQLVF GVVALSPALV ILYSSIVALK RFVKLGNVWS NEKRYREQIS ISLNNVERVL
550 560 570 580 590 600
NYSKQGADAD EEHLNQGLLV IEVSNLYKLG SFLIPRSRKK EWFRDVEELV DTNLDSGAHI
610
NVVNRIYHVY GRFLIH
References and
documentation are available.
Number of amino acids: 616
Molecular weight: 70861.65
Theoretical pI: 6.43
Total number of negatively charged residues (Asp + Glu): 74
Total number of positively charged residues (Arg + Lys): 70
Atomic composition:
Carbon C 3195
Hydrogen H 5072
Nitrogen N 844
Oxygen O 934
Sulfur S 19
Formula: C
3195H
5072N
844O
934S
19
Total number of atoms: 10064
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 78770
Abs 0.1% (=1 g/l) 1.112, assuming all pairs of Cys residues form cystines
Ext. coefficient 78270
Abs 0.1% (=1 g/l) 1.105, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 43.93
This classifies the protein as unstable.
Aliphatic index: 106.88
Grand average of hydropathicity (GRAVY): -0.126