ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MIIRRLFSIS NRSFFLKKPQ FDVKKIIEMI PQYQTSIQNR ELIEADSIIR SLQLLGERYQ 

        70         80         90        100        110        120 
NIKEIDKVIA DIQIQRKSIE AQIKKDKTKI TEYSAALKAL KEQYNDQNSK SSELKKKILE 

       130        140        150        160        170        180 
TCKSLPNTLD PTVPLDAPQI EQWINPLKTH KTSEAQAHVD IMLKKNMLDL QTASNVTGMS 

       190        200        210        220        230        240 
WYYLLNDGAR LEQALVAYAL KKANENGFSS CVPPSITKKE LIDACGFNPR DMNNERQIYA 

       250        260        270        280        290        300 
LQDTNLGLVA TAEIPLAGLG ANKVLELNSG ECSKKLVGVS RCYRAEAGAR GKDTKGLYRV 

       310        320        330        340        350        360 
HEFTKVELFC WSKPETSAKV LEEIKQFQIS VVEELGIPAK VLNMPSNDLG NPAFKKYDIE 

       370        380        390        400        410        420 
AWMPGRGKFG EISSASNCTD FQSRRLNTKY RDDNTGKLEY VHTLNGTAMA IPRVIVALVE 

       430        440 
NFYDPSTGKI SVPECLREFM NGQRYI 

References and documentation are available.

Number of amino acids: 446

Molecular weight: 50389.99

Theoretical pI: 8.92

Amino acid composition: 
Ala (A)  32	  7.2%
Arg (R)  21	  4.7%
Asn (N)  27	  6.1%
Asp (D)  21	  4.7%
Cys (C)   8	  1.8%
Gln (Q)  22	  4.9%
Glu (E)  33	  7.4%
Gly (G)  22	  4.9%
His (H)   4	  0.9%
Ile (I)  35	  7.8%
Leu (L)  42	  9.4%
Lys (K)  42	  9.4%
Met (M)  10	  2.2%
Phe (F)  14	  3.1%
Pro (P)  19	  4.3%
Ser (S)  31	  7.0%
Thr (T)  22	  4.9%
Trp (W)   4	  0.9%
Tyr (Y)  15	  3.4%
Val (V)  22	  4.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 54
Total number of positively charged residues (Arg + Lys): 63

Atomic composition:

Carbon      C	      2236
Hydrogen    H	      3604
Nitrogen    N	       612
Oxygen      O	       672
Sulfur      S	        18

Formula: C2236H3604N612O672S18
Total number of atoms: 7142

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    44850
Abs 0.1% (=1 g/l)   0.890, assuming all pairs of Cys residues form cystines


Ext. coefficient    44350
Abs 0.1% (=1 g/l)   0.880, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 38.08
This classifies the protein as stable.



Aliphatic index: 88.81

Grand average of hydropathicity (GRAVY): -0.423